Cytotoxic effect of ferrimagnetic glass-ceramic nanocomposites on bone osteosarcoma cells

This work pointed out the anti-cancer effect of ferrimagnetic glass ceramic nanocomposites (CaO-ZnO-Fe2O3-SiO2), which contain high amount of magnetite (similar to 60%), crystallite size < 100 nm, and different nucleating agents on bone cancer Saos-2 cells. The cell viability was inhibited by FH and FW to < 50% and < 25%, respectively, with/without magnetism, and both also reduced mitochondrial transmembrane potential (Delta Ym), with/without magnetism (no influence of magnetism). Histone deacetylase (HDAC) activity was inhibited by FH, FW, and FHPNT, with/without magnetism. FHP3/magnetism resulted in HDAC inhibition. In absence of magnetism, FH and FW increased both necrotic and apoptotic cell death, while FW/magnetism induced late apoptosis. DNA fragmentation was increased by FH-and FW-treatment, with/without magnetism. At the same time, FW and FH/magnetism can efficiently induce the intrinsic apoptotic pathway in Saos-2 cells, whereas FW with/without magnetism and FH/magnetism enhanced cytochrome-C release. Similarly, caspase-7 activity was elevated by FH and FW, with/without magnetism. However, the presence of P2O5 in the composition of the nanocomposites inhibited their apoptotic properties and diminished their anti-cancer activity. (C) 2017 Elsevier Masson SAS. All rights reserved.