Molecular characterization of a foot-and-mouth disease virus containing a 57-nucleotide insertion in the 3'untranslated region

被引：9

作者：

Piccone, M. E. ^{[1
,2
]}

Pauszek, S. ^{[1
]}

Pacheco, J. ^{[1
]}

Rieder, E. ^{[1
]}

Kramer, E. ^{[1
]}

Rodriguez, L. L. ^{[1
]}

机构：

[1] ARS, USDA, NAA, Foreign Anim Dis Res Unit,Plum Isl Anim Dis Ctr, Greenport, NY 11944 USA

[2] Univ Connecticut, Dept Pathobiol & Vet Sci, Storrs, CT 06269 USA

来源：

ARCHIVES OF VIROLOGY | 2009年 / 154卷 / 04期

关键词：

RIBOSOME ENTRY SITE; UNTRANSLATED REGION; CELL-FREE; ELEMENT; 3'-NCR;

D O I：

10.1007/s00705-009-0332-x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A foot-and-mouth disease virus containing a 57-nucleotide (nt) insertion in the 3'untranslated region (3'UTR) was generated by transposon (tn)-mediated mutagenesis. Characterization of the mutant virus (A24-3'UTR8110) revealed no significant differences in virus growth, translation efficiency or virulence in cattle compared to the A24 wild-type virus. RNA modeling showed that the structures predicted in the 3'UTR were not affected by the tn insertion. These results revealed that the 3'UTR can tolerate foreign sequences that do not disrupt essential signals required for virus replication.

引用

页码：671 / 676

页数：6

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