Clinical efficacy of Sep-Pak(R) assisted one pot automated synthesis of pharmaceutical grade [18F]FLT using 5′-O-(benzoyl)-2,3′-anhydrothymidine precursor

被引：1

作者：

Mitra, Arpit ^{[1
]}

Chakraborty, Avik ^{[2
]}

Upadhye, Trupti ^{[2
]}

Verma, Priyanka ^{[2
]}

Rajesh, C. ^{[2
]}

Lad, Sangita ^{[2
]}

Pawar, Yogita ^{[2
]}

Basu, Sandip ^{[2
]}

Banerjee, Sharmila ^{[1
,2
]}

机构：

[1] Board Radiat & Isotope Technol, Med Cyclotron Facil, Radiat Med Ctr, Mumbai, Maharashtra, India

[2] Bhabha Atom Res Ctr, Radiat Med Ctr, TMH Annex, Mumbai 400012, Maharashtra, India

来源：

JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY | 2021年 / 327卷 / 01期

关键词：

5 '-O-(Benzoyl)-2,3 ' anhydrothymidine; Radiofluorination; F-18]FLT; Radiochemical; PET/CT; Stavudine; RADIOSYNTHESIS; PET;

D O I：

10.1007/s10967-020-07531-9

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An automated production of pharmaceutical grade 3 '-deoxy-3 '-[F-18]fluorothymidine ([F-18]FLT) with solid phase extraction (SPE) purification using 5 '-O-(Benzoyl)-2,3 ' anhydrothymidine precursor (Benzoyl-Anhydro) poses a major challenge. Herein, an automated, one-pot, two-step, radiochemical synthesis of [F-18]FLT using benzoyl-anhydro precursor and subsequent optimization of SPE purification (using readily available SepPak (R) cartridges) while maintaining radiochemical purity, chemical purity and residual solvent levels within the specified limits has been reported. The current method yields pharmaceutical grade [F-18]FLT suitable for inclusion as a generic product in different pharmacopeia. The desired clinical results add support to indigenously produced Sep-Pak(R) purified [F-18]FLT as a pharmaceutical grade diagnostic radiopharmaceutical.

引用

页码：585 / 596

页数：12

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