Method for direct selection of potentially probiotic Bifidobacterium strains from human feces based on their acid-adaptation ability

被引：46

作者：

Carmen Collado, M. ^{[1
]}

Sanz, Yolanda ^{[1
]}

机构：

[1] CSIC, Inst Agroquim & Tecnol Alimentos, Valencia 46100, Spain

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2006年 / 66卷 / 03期

关键词：

bifidobacterium; probiotics; acid resistance; stress;

D O I：

10.1016/j.mimet.2006.01.007

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method for direct selection of acid-resistant Bifidobacterium strains was developed by prolonged exposure of human feces to homologous lethal stress conditions. The recovered strains were intrinsically resistant to acidic gastric conditions (PH 2.0) and also showed good tolerance to high concentrations of bile salts and NaCl. Bificlobacterium breve and Bifidobacterium adolescentis were, respectively, the infant- and adult-type bificlobacterial species showing the highest ability to develop an acid-tolerant phenotype. Therefore, this procedure could be applicable to the direct selection of Biflidobacterium strains with improved stability in adverse environments and, probably, contribute to expand the spectra of probiotic species of human origin currently marketed. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：560 / 563

页数：4

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