RAPD markers in diversity detection and variety identification of South Tunisian barley

被引:0
|
作者
Guasmi, Ferdaous [1 ]
Marzougui, Nidhal [1 ]
Sarray, Narjes [1 ]
Elfalleh, Walid [1 ]
Ferchichi, Ali [1 ]
机构
[1] Inst Reg Arides, Lab Arido Culture & Cultures Oasiennes, Medenine 4119, Tunisia
来源
JOURNAL OF FOOD AGRICULTURE & ENVIRONMENT | 2009年 / 7卷 / 02期
关键词
Biodiversity; RAPD markers; barley; relationships; GENETIC DIVERSITY; NUCLEAR-DNA; RFLP; ISOZYMES;
D O I
暂无
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
RAPD (Random Amplified Polymorphic DNA) markers were applied to evaluate genetic diversity of South Tunisian barley accessions. Of the 49 fragments generated by 4 selected primers (OPA-04, OPA-18, UBC-490 and UBC-534) 42 appeared to be polymorphic. These RAPD bands were used to calculate Jaccard's distance coefficient for cluster analysis using an arithmetic averaging algorithm (UPGMA). The genetic distance of the 20 studied accessions ranged between 0.25 and 0.68. Of the accessions 39.5% have a wide genetic distance (more than 0.6) and 60.5% are characterized by a distance ranged between 0.3-0.6. The analysis of the hierarchical tree shows the presence of four ramifications thus translating great genetic diversity.
引用
收藏
页码:528 / 533
页数:6
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