The need for a worldwide consensus for cell line authentication: Experience implementing a mandatory requirement at the International Journal of Cancer

被引：39

作者：

Fusenig, Norbert E. ^{[1
]}

Capes-Davis, Amanda ^{[2
]}

Bianchini, Franca ^{[3
]}

Sundell, Sherryl ^{[3
]}

Lichter, Peter ^{[4
,5
]}

机构：

[1] German Canc Res Ctr, Heidelberg, Germany

[2] Univ Sydney, Childrens Med Res Inst, CellBank Australia, Westmead, NSW, Australia

[3] German Canc Res Ctr, Int Journal Canc, Heidelberg, Germany

[4] German Canc Res Ctr, Div Mol Genet, Heidelberg, Germany

[5] German Canc Res Ctr, German Canc Consortium DKTK, Heidelberg, Germany

来源：

PLOS BIOLOGY | 2017年 / 15卷 / 04期

关键词：

CROSS-CONTAMINATION; FALSE; DISCOVERY; CULTURE; CALL;

D O I：

10.1371/journal.pbio.2001438

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cell lines are used in life science research worldwide as biological surrogates. All cell lines are subject to major limitations when used as research tools, including (i) cross-contamination with other cells cultured in the same laboratory environment and (ii) evolution in vitro that renders a given cell line inappropriate as a surrogate for a specific biological hypothesis. There is ample evidence that cross-contamination or phenotypic drift of cells in culture can generate irreproducible or misleading data. A small number of scientific journals-the International Journal of Cancer being at the forefront-and funding agencies have recently moved forward to ask for obligatory cell line authentication data. The history of implementing such rules by the International Journal of Cancer exemplifies the difficulties encountered when installing mandatory quality measures in life sciences.

引用

页数：13

共 35 条

[1]

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[2]

[Anonymous], CHAR CELL LIN COR FA

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