Optimization of Keratinase Production and Utilization of Bacillus pumilus for Feather Degradation

被引：2

作者：

Dhiva, S. ^{[1
]}

Akshara, C. ^{[1
]}

Afna, K. ^{[1
]}

Dhanush, U. ^{[1
]}

Arya, P. ^{[1
]}

Saigeetha, S. ^{[2
]}

Abirami, S. ^{[3
]}

Abraham, Angel ^{[4
]}

Renitta, R. Emilin ^{[4
]}

Samrot, Antony, V ^{[5
]}

机构：

[1] Sree Narayana Coll, Dept Microbiol, Palakkad 678682, Kerala, India

[2] Sathyabama Inst Sci & Technol, Dept Biotechnol, Sch Bio & Chem Engn, Chennai 600119, Tamil Nadu, India

[3] Kamaraj Coll, Dept Micrbiol, Thirunelveli 628003, Tamil Nadu, India

[4] Karunya Inst Technol & Sci, Dept Food Proc, Coimbatore 641114, Tamil Nadu, India

[5] MAHSA Univ, Sch Biosci, Fac Med Biosci & Nursing, Jalan SP2, Bandar Saujana Putra 42610, Selangor, Malaysia

来源：

JOURNAL OF PURE AND APPLIED MICROBIOLOGY | 2020年 / 14卷 / 04期

关键词：

Keratinase; feather; keratinolytic bacteria; SDS-PAGE; CHICKEN FEATHERS; WASTE; SUBSTRATE; PROTEASE;

D O I：

10.22207/JPAM.14.4.26

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Soil samples were collected from the feather dumped area where Bacillus pumilus was isolated and used for keratinase production and keratinolytic activity. In the optimization study, optimal condition for enzyme production was observed at 144 h, pH 7, temperature 37 degrees C. The organism was utilized for feather degradation study. The maximum degradation of 57% was obtained at 37 degrees C, pH 7 and 6 days incubation. The size of keratinase was determined by SDS- PAGE and was observed as 52 KDa.

引用

页码：2483 / 2489

页数：7

共 50 条

[41] Chryseobacterium aquifrigidense keratinase liberated essential and nonessential amino acids from chicken feather degradation
Bokveld, Amahle
Nnolim, Nonso E.
Digban, Tennison O.
Okoh, Anthony I.
Nwodo, Uchechukwu U.
ENVIRONMENTAL TECHNOLOGY, 2023, 44 (03) : 293 - 303
[42] Production of Keratinase from a Newly Isolated Feather Degrading Bacillus cereus VITSDVM4 from Poultry Waste
Devi, C. Subathra
Shankar, Ravi
Kumar, Sanjeev
Mohanasrinivasan, V.
Vaishnavi, B.
NATIONAL ACADEMY SCIENCE LETTERS-INDIA, 2018, 41 (05): : 307 - 311
[43] Production of Keratinase from a Newly Isolated Feather Degrading Bacillus cereus VITSDVM4 from Poultry Waste
C. Subathra Devi
Ravi Shankar
Sanjeev Kumar
V. Mohanasrinivasan
B. Vaishnavi
National Academy Science Letters, 2018, 41 : 307 - 311
[44] Isolation of keratinase-producing Bacillus strains and enhanced enzyme production using in vitro mutagenesis
Karadagli, Meryem
Ozcan, Bahri Devrim
ANAIS DA ACADEMIA BRASILEIRA DE CIENCIAS, 2022, 94 (01):
[45] Directed evolution driving the generation of an efficient keratinase variant to facilitate the feather degradation
Jing Zhang
Chang Su
Xiao-Li Kong
Jin-Song Gong
Yan-Lin Liu
Heng Li
Jiufu Qin
Zheng-Hong Xu
Jin-Song Shi
Bioresources and Bioprocessing, 9
[46] Directed evolution driving the generation of an efficient keratinase variant to facilitate the feather degradation
Zhang, Jing
Su, Chang
Kong, Xiao-Li
Gong, Jin-Song
Liu, Yan-Lin
Li, Heng
Qin, Jiufu
Xu, Zheng-Hong
Shi, Jin-Song
BIORESOURCES AND BIOPROCESSING, 2022, 9 (01)
[47] Bioutilization of poultry feather for keratinase production and its application in leather industry
Kalaikumari, S. Santha
Vennila, T.
Monika, V.
Chandraraj, K.
Gunasekaran, P.
Rajendhran, J.
JOURNAL OF CLEANER PRODUCTION, 2019, 208 : 44 - 53
[48] A Novel Thermostable Keratinase from Deinococcus geothermalis with Potential Application in Feather Degradation
Tang, Yin
Guo, Leizhou
Zhao, Mingming
Gui, Yuan
Han, Jiahui
Lu, Wei
Dai, Qilin
Jiang, Shijie
Lin, Min
Zhou, Zhengfu
Wang, Jin
APPLIED SCIENCES-BASEL, 2021, 11 (07):
[49] Feather degradation by Bacillus sp FK 46 in submerged cultivation
Suntornsuk, W
Suntornsuk, L
BIORESOURCE TECHNOLOGY, 2003, 86 (03) : 239 - 243
[50] Microbial production of keratinase from Bacillus velezensis strain MAMA: A novel enzyme for eco-friendly degradation of keratin waste
Khan, Aimon
Mehmood, Kashif
Nadhman, Akhtar
Khan, Sami Ullah
Shah, Aamer Ali
Shah, Ziaullah
HELIYON, 2024, 10 (12)

← 1 2 3 4 5 →