Optimization of Keratinase Production and Utilization of Bacillus pumilus for Feather Degradation

被引：2

作者：

Dhiva, S. ^{[1
]}

Akshara, C. ^{[1
]}

Afna, K. ^{[1
]}

Dhanush, U. ^{[1
]}

Arya, P. ^{[1
]}

Saigeetha, S. ^{[2
]}

Abirami, S. ^{[3
]}

Abraham, Angel ^{[4
]}

Renitta, R. Emilin ^{[4
]}

Samrot, Antony, V ^{[5
]}

机构：

[1] Sree Narayana Coll, Dept Microbiol, Palakkad 678682, Kerala, India

[2] Sathyabama Inst Sci & Technol, Dept Biotechnol, Sch Bio & Chem Engn, Chennai 600119, Tamil Nadu, India

[3] Kamaraj Coll, Dept Micrbiol, Thirunelveli 628003, Tamil Nadu, India

[4] Karunya Inst Technol & Sci, Dept Food Proc, Coimbatore 641114, Tamil Nadu, India

[5] MAHSA Univ, Sch Biosci, Fac Med Biosci & Nursing, Jalan SP2, Bandar Saujana Putra 42610, Selangor, Malaysia

来源：

JOURNAL OF PURE AND APPLIED MICROBIOLOGY | 2020年 / 14卷 / 04期

关键词：

Keratinase; feather; keratinolytic bacteria; SDS-PAGE; CHICKEN FEATHERS; WASTE; SUBSTRATE; PROTEASE;

D O I：

10.22207/JPAM.14.4.26

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Soil samples were collected from the feather dumped area where Bacillus pumilus was isolated and used for keratinase production and keratinolytic activity. In the optimization study, optimal condition for enzyme production was observed at 144 h, pH 7, temperature 37 degrees C. The organism was utilized for feather degradation study. The maximum degradation of 57% was obtained at 37 degrees C, pH 7 and 6 days incubation. The size of keratinase was determined by SDS- PAGE and was observed as 52 KDa.

引用

页码：2483 / 2489

页数：7

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