A study of host preference in tsetse flies using a modified heteroduplex PCR-based method

被引：32

作者：

Njiokou, F

Simo, G

Mbida, AM

Truc, P

Cuny, G

Herder, S

机构：

[1] OCEAC, Lab Rech Trypanosomoses, Yaounde, Cameroon

[2] Univ Yaounde, Fac Sci, Dept BPA, Yaounde, Cameroon

[3] Inst Rech Med & Etud Plantes Med, Yaounde, Cameroon

[4] Inst Rech Dev, Yaounde, Cameroon

[5] CIRAD, IRD, Lab Rech & Coordinat Trypanosomoses, F-34398 Montpellier 5, France

来源：

ACTA TROPICA | 2004年 / 91卷 / 02期

关键词：

Glossina spp; bloodmeals; PCR; heteroduplex analysis; wild animals;

D O I：

10.1016/j.actatropica.2004.03.006

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

A study of host preference in tsetse flies using a modified heteroduplex. PCR-based method is described. Domestic and wild animal blood samples were collected to extract the corresponding reference DNAs. In Campo (south Cameroon), tsetse flies (mainly Glossina palpalis palpalis) were trapped and 41 bloodmeals were collected. All reference DNAs and 37 bloodmeal DNAs (90.7%) were successfully amplified and hybridised. Twelve bloodmeals (32.4%) were of human origin, 13 (35.4%) were from Sitatunga (Tragelaphus spekei) (an antelope) while 12 (32.4%) were not identified using our set of reference DNAs. The results confirmed the occurrence of frequent contacts between wild animals and this population of tsetse flies. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：117 / 120

页数：4

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