Kinetics of membrane binding and dissociation of 5-fluorouracil by pulsed-field-gradient 19F NMR

被引：7
|
作者：
Yoshii, Noriyuki [1 ]
Okamura, Emiko [1 ]
机构：
[1] Himeji Dokkyo Univ, Fac Pharmaceut Sci, Himeji, Hyogo 6708524, Japan
来源：
CHEMICAL PHYSICS LETTERS | 2009年 / 474卷 / 4-6期
基金：
日本学术振兴会;
关键词：
PHOSPHOLIPID-BILAYER MEMBRANES; DIFFUSION EXPERIMENTS; CHEMICAL-EXCHANGE; SPECTRA; RATES;
D O I：
10.1016/j.cplett.2009.04.078
中图分类号：
O64 [物理化学（理论化学）、化学物理学];
学科分类号：
070304 ; 081704 ;
摘要：
The kinetics of membrane binding and dissociation of an anticancer drug, 5-fluorouracil (5FU) is quantified by high resolution NMR with the pulsed-field-gradient technique. The F-19 NMR signal of 5FU is analyzed at 293-313 K by the solution of Bloch equation with exchange terms. The rate constants of 5FU binding and dissociation are 0.2 and 4.1 s (1) at 303 K. The 5FU motion in the vertical direction to the membrane surface is restricted as compared with the lateral diffusion, judging from the activation energy (57 kJ/mol) larger than the lateral diffusion in membrane (26 kJ/mol [E. Okamura, N. Yoshii, J. Chem. Phys. 129 (2008) 215102]). (C) 2009 Elsevier B.V. All rights reserved.
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页码：357 / 361
页数：5
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