ISSR and AFLP markers differ among American watermelon cultivars with limited genetic diversity

被引：59
作者：
Levi, A
Thomas, CE
Newman, M
Reddy, OUK
Zhang, X
Xu, Y
机构：
[1] USDA ARS, US Vegetable Lab, Charleston, SC 29414 USA
[2] USDA ARS, Plant Genet Resources & Conservat Unit, Griffin, GA 30223 USA
[3] Alcorn State Univ, Ctr Plant Biotechnol & Genom, Lorman, MS 39096 USA
[4] Syngenta Seeds, Woodland, CA 95695 USA
[5] Natl Engn Res Ctr Vegetables, Beijing 100089, Peoples R China
来源：
JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE | 2004年 / 129卷 / 04期
关键词：
RAPD; DNA markers; germplasm evaluation; vegetable breeding; Citrullus lanatus;
D O I：
10.21273/JASHS.129.4.0553
中图分类号：
S6 [园艺];
学科分类号：
0902 ;
摘要：
Wide phenotypic diversity exists among American heirloom cultivars of watermelon (Citrullus lanatus var. lanatus). However, in published studies, low or no polymorphism was revealed among those heirlooms using isozyme or randomly amplified polymorphic DNA (RAPD) markers. In this study, experiments with inter-simple sequence repeat (ISSR) [also known as simple sequence repeat-(SSR-) anchored primers] and amplified fragment-length polymorphism (AFLP) markers produced high polymorphisms among watermelon heirloom cultivars. ISSR (111) and AFLP (118) markers (229 total) identified 80.2% to 97.8% genetic similarity among heirloom cultivars. The phylogenetic relations based on ISSR and AFLP markers are highly consistent with the parental records available for some of the heirloom cultivars, providing confidence in the dendogram constructed for heirlooms based on similarity values. As compared with RAPD markers, ISSRs and AFLPs are highly effective in differentiating among watermelon cultivars or elite lines with limited genetic diversity.
引用
收藏
页码：553 / 558
页数：6
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