CRISPR-based genome editing in wheat: a comprehensive review and future prospects

被引:34
作者
Kumar, Rakesh [1 ]
Kaur, Amandeep [1 ]
Pandey, Ankita [1 ,2 ]
Mamrutha, H. M. [1 ]
Singh, G. P. [1 ]
机构
[1] IIWBR, ICAR, Karnal 132001, Haryana, India
[2] Banasthali Vidyapith, Biosci & Biotechnol, Jaipur 304022, Rajasthan, India
关键词
CRISPR; Wheat; SgRNA; Cas9; Cpf1; Agrobacterium; Biolistic; Protoplast; Knockout; FREQUENCY TARGETED MUTAGENESIS; RNA-GUIDED ENDONUCLEASE; DROUGHT STRESS; RICE; SYSTEM; PLANTS; EFFICIENT; DNA; CAS9; NUCLEASES;
D O I
10.1007/s11033-019-04761-3
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
CRISPR technology has vividly increased its applications in last five years for genome editing in a wide range of organisms from bacteria to plants. It is mostly applied in the field of mammalian research. This emerging versatile tool can be utilized in crop improvement by targeting various traits to increase economic value and adaptability of the crop species under changing climate. In plants, Arabidopsis and rice are the most studied plant species in genome editing through CRISPR technology. Wheat is lagging behind in the utilization of CRISPR based genome modifications. The hexaploid, large genome size and the recalcitrant nature in terms of tissue culture are the major obstacles for CRISPR utilization in wheat. Recently, the IWGSC released the high quality of reference genome for wheat which will greatly accelerate the application of CRISPR-based genome engineering in wheat and helps to resolve the global issue of food security in coming decades. The exogenous DNA-free improved mutants with CRISPR technology having desired traits will increase the productivity under biotic and abiotic stress conditions. To address complex traits involving multigene, recently developed multiplex genome editing toolkits can be used. This is a first review of its kind in which the practical utilization and updates on CRISPR validation in wheat along with its future prospects for use of this technology in wheat improvement are comprehensively discussed. Thus, the compiled information will immensely benefit the researchers for utilization of CRISPR system in wheat improvement across the globe.
引用
收藏
页码:3557 / 3569
页数:13
相关论文
共 86 条
[1]   Genome editing for crop improvement: Challenges and opportunities [J].
Abdallah, Naglaa A. ;
Prakash, Channapatna S. ;
McHughen, Alan G. .
GM CROPS & FOOD-BIOTECHNOLOGY IN AGRICULTURE AND THE FOOD CHAIN, 2015, 6 (04) :183-205
[2]   Efficient Virus-Mediated Genome Editing in Plants Using the CRISPR/Cas9 System [J].
Ali, Zahir ;
Abul-faraj, Aala ;
Li, Lixin ;
Ghosh, Neha ;
Piatek, Marek ;
Mahjoub, Ali ;
Aouida, Mustapha ;
Piatek, Agnieszka ;
Baltes, Nicholas J. ;
Voytas, Daniel F. ;
Dinesh-Kumar, Savithramma ;
Mahfouz, Magdy M. .
MOLECULAR PLANT, 2015, 8 (08) :1288-1291
[3]   Structural Plasticity of PAM Recognition by Engineered Variants of the RNA-Guided Endonuclease Cas9 [J].
Anders, Carolin ;
Bargsten, Katja ;
Jinek, Martin .
MOLECULAR CELL, 2016, 61 (06) :895-902
[4]   Shifting the limits in wheat research and breeding using a fully annotated reference genome [J].
Appels, Rudi ;
Eversole, Kellye ;
Feuillet, Catherine ;
Keller, Beat ;
Rogers, Jane ;
Stein, Nils ;
Pozniak, Curtis J. ;
Choulet, Frederic ;
Distelfeld, Assaf ;
Poland, Jesse ;
Ronen, Gil ;
Sharpe, Andrew G. ;
Pozniak, Curtis ;
Barad, Omer ;
Baruch, Kobi ;
Keeble-Gagnere, Gabriel ;
Mascher, Martin ;
Ben-Zvi, Gil ;
Josselin, Ambre-Aurore ;
Himmelbach, Axel ;
Balfourier, Francois ;
Gutierrez-Gonzalez, Juan ;
Hayden, Matthew ;
Koh, ChuShin ;
Muehlbauer, Gary ;
Pasam, Raj K. ;
Paux, Etienne ;
Rigault, Philippe ;
Tibbits, Josquin ;
Tiwari, Vijay ;
Spannagl, Manuel ;
Lang, Daniel ;
Gundlach, Heidrun ;
Haberer, Georg ;
Mayer, Klaus F. X. ;
Ormanbekova, Danara ;
Prade, Verena ;
Simkova, Hana ;
Wicker, Thomas ;
Swarbreck, David ;
Rimbert, Helene ;
Felder, Marius ;
Guilhot, Nicolas ;
Kaithakottil, Gemy ;
Keilwagen, Jens ;
Leroy, Philippe ;
Lux, Thomas ;
Twardziok, Sven ;
Venturini, Luca ;
Juhasz, Angela .
SCIENCE, 2018, 361 (6403) :661-+
[5]   Gene Editing and Crop Improvement Using CRISPR-Cas9 System [J].
Arora, Leena ;
Narula, Alka .
FRONTIERS IN PLANT SCIENCE, 2017, 8
[6]   Targeted mutagenesis in wheat microspores using CRISPR/Cas9 [J].
Bhowmik, Pankaj ;
Ellison, Evan ;
Polley, Brittany ;
Bollina, Venkatesh ;
Kulkarni, Manoj ;
Ghanbarnia, Kaveh ;
Song, Halim ;
Gao, Caixia ;
Voytas, Daniel F. ;
Kagale, Sateesh .
SCIENTIFIC REPORTS, 2018, 8
[7]   The CRISPR/Cas9 system for plant genome editing and beyond [J].
Bortesi, Luisa ;
Fischer, Rainer .
BIOTECHNOLOGY ADVANCES, 2015, 33 (01) :41-52
[8]  
Brandt K.M., 2017, 13 IWGS TULLN
[9]   A Multipurpose Toolkit to Enable Advanced Genome Engineering in Plants [J].
Cermak, Tomas ;
Curtin, Shaun J. ;
Gil-Humanes, Javier ;
Cegan, Radim ;
Kono, Thomas J. Y. ;
Konecna, Eva ;
Belanto, Joseph J. ;
Starker, Colby G. ;
Mathre, Jade W. ;
Greenstein, Rebecca L. ;
Voytas, Daniel F. .
PLANT CELL, 2017, 29 (06) :1196-1217
[10]   An Agrobacterium-delivered CRISPR/Cas9 system for high-frequency targeted mutagenesis in maize [J].
Char, Si Nian ;
Neelakandan, Anjanasree K. ;
Nahampun, Hartinio ;
Frame, Bronwyn ;
Main, Marcy ;
Spalding, Martin H. ;
Becraft, Philip W. ;
Meyers, Blake C. ;
Walbot, Virginia ;
Wang, Kan ;
Yang, Bing .
PLANT BIOTECHNOLOGY JOURNAL, 2017, 15 (02) :257-268