Population structure and genetic diversity of Vibrio parahaemolyticus from a coastal area of China based on a multi-locus sequence typing (MLST) scheme

被引:6
作者
Li, Yu [1 ,2 ,3 ]
Yin, Hong-Qiu [4 ]
Xia, Jun [5 ]
Luo, Hong [3 ]
Wang, Ming-Yi [2 ]
机构
[1] Qingdao Univ, Yuhuangding Hosp, Dept Clin, Lab Ctr, Yantai 264000, Shandong, Peoples R China
[2] Dalian Med Univ, Weihai Municipal Hosp, Dept Cent Lab, Weihai 264200, Shandong, Peoples R China
[3] Dalian Med Univ, Coll Lab Med, Dept Lab Med, Dalian 116044, Liaoning, Peoples R China
[4] Wendeng Osteopath Hosp, Weihai 264400, Shandong, Peoples R China
[5] Weihai Hlth & Family Planning Commiss, Dept Med Adm, Weihai 264200, Shandong, Peoples R China
来源
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY | 2019年 / 112卷 / 08期
关键词
Clonal complex; Genetic diversity; MLST; Population structure; Vibrio parahaemolyticus; STRAINS; O3-K6;
D O I
10.1007/s10482-019-01252-0
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The Gram-negative marine bacterium Vibrio parahaemolyticus has been identified as a major cause of bacterial food poisoning in China. Here, the population structure and genetic diversity of V. parahaemolyticus from Weihai, a coastal city in China, was studied by the multi-locus sequence typing (MLST) method. In this survey, we isolated 40 strains including environmental and clinical samples of patients with acute gastroenteritis or diarrhea; isolates from other countries were also included for comparison. DnaSP Version5, START V2, eBURST version3 and Mega 6 were used to analyse the data. We found that ST3 and ST332 were the most prevalent clones and that they were closely associated with acute diarrhoeal diseases. These STs showed a low d(N)/d(S) ratio and significant linkage disequilibrium. All isolates were divided into four clonal complexes, six groups and nine singletons, showing a high degree of genetic diversity. 18 STs, mostly from environmental isolates, were recognised by the MLST analysis for the first time. In conclusion, ST3 and ST332 were the epidemic STs in the coastal area. ST332 might be a region-specific ST, which needs to be confirmed by further analysis. Thus, the long-term monitoring of V. parahaemolyticus plays an important role in preventing and controlling the transmission between environment and people in Weihai.
引用
收藏
页码:1199 / 1211
页数:13
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