Nucleoli in a pronuclei-stage mouse embryo are represented by major satellite DNA of interconnecting chromosomes

被引：17
作者：
Dozortsev, D
Coleman, A
Nagy, P
Diamond, MP
Ermilov, A
Weier, U
Liyanage, M
Reid, T
机构：
[1] Wayne State Univ, Dept Obstet & Gynecol, Div Reprod Endocrinol, Detroit, MI USA
[2] European Hosp, Rome, Italy
[3] Univ Calif Berkeley, Lawrence Berkeley Lab, Berkeley, CA 94720 USA
[4] NCI, Genet Lab, Div Basic Sci, Bethesda, MD 20892 USA
[5] NIH, Genome Technol Branch, Natl Human Genome Res Inst, Bethesda, MD 20892 USA
来源：
FERTILITY AND STERILITY | 2000年 / 73卷 / 02期
关键词：
zygote; nucleoli; alpha-satellite; centromeres; telomeres;
D O I：
10.1016/S0015-0282(99)00491-4
中图分类号：
R71 [妇产科学];
学科分类号：
100211 ;
摘要：
Objective: To investigate the arrangement of chromosomes within pronuclei-stage mouse zygotes. Design: In vitro study. Setting: Academic medical center. Patient(s): None. Intervention(s): None. Main Outcome Measure(s): Location of major alpha-satellite DNA, centromeres, and telomeres, and relative location of chromosomes. Result(s): Chromosomes appeared to be oriented inward by centromeres and to be interconnected by major alpha-satellite DNA, which appeared to be the sole DNA component of the nucleoli. This chromosomal arrangement persisted throughout interphase. Chromosomal painting failed to identify chromosomal ordering within pronuclei. Conclusion(s): Pronuclear nucleoli are represented by alpha-satellite sequences of interconnecting chromosomes that hold all chromosomes together during interphase. Chromosomes within the pronucleus are randomly positioned relative to each other. (Fertil Steril(R) 2000;73:366-71. (C)2000 by American Society for Reproductive Medicine.).
引用
收藏
页码：366 / 371
页数：6
相关论文
共 19 条
[1]   ARRANGEMENT OF CHROMATIN IN THE NUCLEUS [J].
COMINGS, DE .
HUMAN GENETICS, 1980, 53 (02) :131-143
[2]   Ndj1p, a meiotic telomere protein required for normal chromosome synapsis and segregation in yeast [J].
Conrad, MN ;
Dominguez, AM ;
Dresser, ME .
SCIENCE, 1997, 276 (5316) :1252-1255
[3]   CENTROMERIC ASSOCIATION AND NON-RANDOM DISTRIBUTION OF CENTROMERES IN HUMAN-TUMOR CELLS [J].
HAAF, T ;
SCHMID, M .
HUMAN GENETICS, 1989, 81 (02) :137-143
[4]   DIRECT EVIDENCE FOR THE NONRANDOM LOCALIZATION OF MAMMALIAN CHROMOSOMES IN THE INTERPHASE NUCLEUS [J].
HADLACZKY, G ;
WENT, M ;
RINGERTZ, NR .
EXPERIMENTAL CELL RESEARCH, 1986, 167 (01) :1-15
[5]   Regulation of meiotic chromatin loop size by chromosomal position [J].
Heng, HHQ ;
Chamberlain, JW ;
Shi, XM ;
Spyropoulos, B ;
Tsui, LC ;
Moens, PB .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (07) :2795-2800
[6]  
KATSUMATA M, 1988, J CELL SCI, V90, P193
[7]   Multicolour spectral karyotyping of mouse chromosomes [J].
Liyanage, M ;
Coleman, A ;
duManoir, S ;
Veldman, T ;
McCormack, S ;
Dickson, RB ;
Barlow, C ;
WynshawBoris, A ;
Janz, S ;
Wienberg, J ;
FergusonSmith, MA ;
Schrock, E ;
Ried, T .
NATURE GENETICS, 1996, 14 (03) :312-315
[8]  
MANIOTIS AG, 1997, J CELL BIOCHEM, V64, P1
[9]   DIFFERENT CENTRAL NERVOUS-SYSTEM CELL-TYPES DISPLAY DISTINCT AND NONRANDOM ARRANGEMENTS OF SATELLITE DNA-SEQUENCES [J].
MANUELIDIS, L .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (10) :3123-3127
[10]   REPRODUCIBLE COMPARTMENTALIZATION OF INDIVIDUAL CHROMOSOME DOMAINS IN HUMAN CNS CELLS REVEALED BY INSITU HYBRIDIZATION AND 3-DIMENSIONAL RECONSTRUCTION [J].
MANUELIDIS, L ;
BORDEN, J .
CHROMOSOMA, 1988, 96 (06) :397-410
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