High Genetic Diversity of Anaplasma marginale Detected from Philippine Cattle

被引：26
作者：
Patalinghug Ybanez, Adrian [1 ,2 ,3 ]
Ybanez, Rochelle Haidee D. [3 ]
Claveria, Florencia G. [4 ]
Jane Cruz-Flores, Mary [4 ]
Xuenan, Xuen
Yokoyama, Naoaki [2 ]
Inokuma, Hisashi [1 ,2 ]
机构：
[1] Obihiro Univ Agr & Vet Med, Dept Vet Clin Sci, Obihiro, Hokkaido 0808555, Japan
[2] Gifu Univ, United Grad Sch Vet Sci, Gifu 5011193, Japan
[3] Visayas State Univ Visca, Baybay City 6521 A, Leyte, Philippines
[4] De La Salle Univ, Coll Sci, Dept Biol, Manila 1000, Philippines
来源：
JOURNAL OF VETERINARY MEDICAL SCIENCE | 2014年 / 76卷 / 07期
关键词：
Anaplasma marginale; cattle; groEL; Mspla; Philippines; RHIPICEPHALUS BOOPHILUS MICROPLUS; VECTOR-BORNE PATHOGENS; SURFACE PROTEIN 1A; TRANSMISSION; INFECTIONS; CONSERVATION; EHRLICHIA; TICKS; CEBU; HERD;
D O I：
10.1292/jvms.13-0405
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
A total of 658 cattle in 6 provinces in the Philippines were screened for Anaplasma marginate infection by using a diagnostic heat-shock operon (groEL) gene-PCR assay. The screening-positive samples were further tested using the major surface antigen protein la (Mspla) gene-PCR assay. Screening PCR results showed 130 cattle (19.8%) were positive for the A. marginale infection. Subsequent amplification using the Mspla gene only showed 93 samples (14.1%) to be positive. In addition, 37 tandem-repeat structures, including 20 novel structures, and 41 distinct genotypes were identified. Interestingly, multiple infections of 4 different genotypes were also observed in A. marginale-infected cattle. The present study demonstrated the prevalence and characterization of diverse genotypes of A. marginale in the Philippine cattle.
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收藏
页码：1009 / 1014
页数：6
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