Pollen Transmission of Asparagus virus 2 (AV-2) May Facilitate Mixed Infection by Two AV-2 Isolates in Asparagus Plants

被引：8

作者：

Kawamura, Ryusuke ^{[1
]}

Shimura, Hanako ^{[1
]}

Mochizuki, Tomofumi ^{[2
]}

Ohki, Satoshi T. ^{[2
]}

Masuta, Chikara ^{[1
]}

机构：

[1] Hokkaido Univ, Grad Sch Agr, Sapporo, Hokkaido 0608589, Japan

[2] Osaka Prefecture Univ, Grad Sch Life & Environm Sci, Sakai, Osaka 591, Japan

来源：

PHYTOPATHOLOGY | 2014年 / 104卷 / 09期

关键词：

CUCUMBER-MOSAIC-VIRUS; NATURAL-POPULATIONS; SEED TRANSMISSION; RECOMBINATION; INVASION;

D O I：

10.1094/PHYTO-12-13-0348-R

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Asparagus virus 2 (AV-2) is a member of the genus Ilarvirus and thought to induce the asparagus decline syndrome. AV-2 is known to be transmitted by seed, and the possibility of pollen transmission was proposed 25 years ago but not verified. In AV-2 sequence analyses, we have unexpectedly found mixed infection by two distinct AV-2 isolates in two asparagus plants. Because mixed infections by two related viruses are normally prevented by cross protection, we suspected that pollen transmission of AV-2 is involved in mixed infection. Immunohistochemical analyses and in situ hybridization using AV-2-infected tobacco plants revealed that AV-2 was localized in the meristem and associated with pollen grains. To experimentally produce a mixed infection via pollen transmission, two Nicotiana benthamiana plants that were infected with each of two AV-2 isolates were crossed. Derived cleaved-amplified polymorphic sequence analysis identified each AV-2 isolate in the progeny seedlings, suggesting that pollen transmission could indeed result in a mixed infection, at least in N. benthamiana.

引用

页码：1001 / 1006

页数：6