Isolation and characterization of novel α-amylase from marine Streptomyces sp D1

被引：83

作者：

Chakraborty, Samrat ^{[1
]}

Khopade, Abhijit ^{[1
]}

Kokare, Chandrakant ^{[1
]}

Mahadik, Kakasaheb ^{[1
]}

Chopade, Balasaheb ^{[2
]}

机构：

[1] Bharati Vidyapeeth Univ, Dept Pharmaceut Biotechnol, Poona Coll Pharm, Pune 411038, Maharashtra, India

[2] Univ Pune, Inst Bioinformat & Biotechnol, Pune 411007, Maharashtra, India

来源：

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC | 2009年 / 58卷 / 1-4期

关键词：

Marine; Streptomyces; Halophilic; Surfactant; Detergent stable; ALKALINE PROTEASE; BACILLUS SP; GEN; NOV; PURIFICATION;

D O I：

10.1016/j.molcatb.2008.10.011

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this study, we have reported novel alpha-amylase enzyme from less extensively studied marine Streptomyces sp. D1. Enzyme production was determined by using media containing 2% sucrose. 0.35% peptone and 0.15% of malt extract. Optimum temperature for enzyme production and activity was found to be 45 degrees C and enzyme retained almost 50% of its activity at 85 degrees C. Enzyme activity was also retained in presence of commercially available detergent and oxidizing agents. The partially purified enzyme from strain D1 exhibited specific activity of 113.64 U/mg protein that corresponds to 2.8-fold Purification. SDS-PAGE and zymograin activity staining showed a single band equal to molecular mass of 66 kDa. The reported enzyme may have wide spread application for detergent and pharmaceutical industry. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：17 / 23

页数：7

共 36 条

[1] KINETICS OF ALPHA-AMYLASE PRODUCTION IN A BATCH AND A FED-BATCH CULTURE OF BACILLUS-SUBTILIS [J].

BAIG, MA ;

PAZLAROVA, J ;

VOTRUBA, J .

FOLIA MICROBIOLOGICA, 1984, 29 (05) :359-364

[2]

CARBAJAL AF, 2002, WORLD J MICROB BIOT, V18, P791

[3]

Chandramohan D, 1997, J MAR BIOTECHNOL, V5, P73

[4]

Chandrasekaran M, 1997, J MAR BIOTECHNOL, V5, P86

[5]

Cordeiro CAM, 2002, BRAZ J MICROBIOL, V33, P57, DOI 10.1590/S1517-83822002000100012

[6] Production and biochemical characterization of an α-amylase from the moderate halophile Halomonas meridiana [J].

Coronado, MJ ;

Vargas, C ;

Hofemeister, J ;

Ventosa, A ;

Nieto, JJ .

FEMS MICROBIOLOGY LETTERS, 2000, 183 (01) :67-71

[7] Characterization of a salt-tolerant extracellular a-amylase from Bacillus dipsosauri [J].

Deutch, CE .

LETTERS IN APPLIED MICROBIOLOGY, 2002, 35 (01) :78-84

[8] Biotechnological uses of archaeal extremozymes [J].

Eichler, J .

BIOTECHNOLOGY ADVANCES, 2001, 19 (04) :261-278

[9]

Gupta MN, 2002, INDIAN J BIOCHEM BIO, V39, P220

[10] A PERSPECTIVE ON THE BIOTECHNOLOGICAL POTENTIAL OF EXTREMOPHILES [J].

HERBERT, RA .

TRENDS IN BIOTECHNOLOGY, 1992, 10 (11) :395-402

← 1 2 3 4 →