Best practices and benchmarks for intact protein analysis for top-down mass spectrometry

被引：234

作者：

Donnelly, Daniel P. ^{[1
,2
,3
]}

Rawlins, Catherine M. ^{[1
,2
,3
]}

DeHart, Caroline J. ^{[4
,5
]}

Fornelli, Luca ^{[4
,5
]}

Schachner, Luis F. ^{[4
,5
]}

Lin, Ziqing ^{[6
]}

Lippens, Jennifer L. ^{[7
]}

Aluri, Krishna C. ^{[1
,2
,3
,8
]}

Sarin, Richa ^{[1
,2
,3
,9
]}

Chen, Bifan ^{[6
]}

Lantz, Carter ^{[10
,11
]}

Jung, Wonhyeuk ^{[10
,11
]}

Johnson, Kendall R. ^{[1
,2
,3
]}

Koller, Antonius ^{[1
,2
,3
]}

Wolff, Jeremy J. ^{[12
]}

Campuzano, Iain D. G. ^{[7
]}

Auclair, Jared R. ^{[13
]}

Ivanov, Alexander R. ^{[1
,2
,3
]}

Whitelegge, Julian P. ^{[14
]}

Pasa-Tolic, Ljiljana ^{[15
]}

Chamot-Rooke, Julia ^{[16
]}

Danis, Paul O. ^{[17
]}

Smith, Lloyd M. ^{[18
]}

Tsybin, Yury O. ^{[19
]}

Loo, Joseph A. ^{[10
,11
]}

Ge, Ying ^{[6
]}

Kelleher, Neil L. ^{[4
,5
]}

Agar, Jeffrey N. ^{[1
,2
,3
]}

机构：

[1] Northeastern Univ, Barnett Inst Chem & Biol Anal, Boston, MA 02115 USA

[2] Northeastern Univ, Dept Chem & Chem Biol, Boston, MA 02115 USA

[3] Northeastern Univ, Dept Pharmaceut Sci, Boston, MA 02115 USA

[4] Northwestern Univ, Dept Chem & Mol Biosci, Evanston, IL USA

[5] Northwestern Univ, Prote Ctr Excellence, Evanston, IL USA

[6] Univ Wisconsin, Dept Chem, Dept Cell & Regenerat Biol, Human Prote Program, 1101 Univ Ave, Madison, WI 53706 USA

[7] Amgen Inc, Amgen Res Discovery Attribute Sci, Thousand Oaks, CA USA

[8] Alnylam Pharmaceut, Cambridge, MA USA

[9] Biogen, Cambridge, MA USA

[10] Univ Calif Los Angeles, Dept Biol Chem, Dept Chem & Biochem, Los Angeles, CA 90024 USA

[11] Univ Calif Los Angeles, DOE Inst Genom & Prote, Los Angeles, CA USA

[12] Bruker Daltonics Inc, Billerica, MA USA

[13] Northeastern Univ, Biopharmaceut Anal Training Lab, Burlington, MA USA

[14] Univ Calif Los Angeles, David Geffen Sch Med, Pasarow Mass Spectrometry Lab, Jane & Terry Semel Inst Neurosci & Human Behav, Los Angeles, CA 90095 USA

[15] Pacific Northwest Natl Lab, Environm Mol Sci Lab, Richland, WA 99352 USA

[16] Inst Pasteur, USR 2000, CNRS, Mass Spectrometry Biol Unit, Paris, France

[17] Eastwoods Consulting, Boylston, MA USA

[18] Univ Wisconsin, Dept Chem, Genome Ctr Wisconsin, 1101 Univ Ave, Madison, WI 53706 USA

[19] Spectroswiss, Lausanne, Switzerland

来源：

NATURE METHODS | 2019年 / 16卷 / 07期

关键词：

INTEGRAL MEMBRANE-PROTEINS; ELECTROSPRAY-IONIZATION; HIGH-RESOLUTION; PROTEOMICS; PROTEOFORM; ELECTROPHORESIS; MOLECULES; ARTIFACTS; MECHANISM; RECOVERY;

D O I：

10.1038/s41592-019-0457-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

One gene can give rise to many functionally distinct proteoforms, each of which has a characteristic molecular mass. Top-down mass spectrometry enables the analysis of intact proteins and proteoforms. Here members of the Consortium for Top-Down Proteomics provide a decision tree that guides researchers to robust protocols for mass analysis of intact proteins (antibodies, membrane proteins and others) from mixtures of varying complexity. We also present cross-platform analytical benchmarks using a protein standard sample, to allow users to gauge their proficiency.

引用

页码：587 / 594

页数：8

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