Characterization of nitrifying microbial community in a submerged membrane bioreactor at short solids retention times

被引：23
作者：
Duan, Liang [1 ,2 ]
Song, Yonghui [1 ]
Xia, Siqing [3 ]
Hermanowicz, Slawomir W. [2 ,4 ]
机构：
[1] Chinese Res Inst Environm Sci, State Key Lab Environm Criteria & Risk Assessment, Beijing 100012, Peoples R China
[2] Univ Calif Berkeley, Dept Civil & Environm Engn, Berkeley, CA 94720 USA
[3] Tongji Univ, State Key Lab Pollut Control & Resource Reuse, Shanghai 200092, Peoples R China
[4] Tongji Univ, Natl High End Foreign Experts China Program, Shanghai 200092, Peoples R China
来源：
BIORESOURCE TECHNOLOGY | 2013年 / 149卷
基金：
中国国家自然科学基金;
关键词：
Membrane bioreactor; Short solids retention time (SRT); Ammonia-oxidizing bacteria (AOB); Nitrite-oxidizing bacteria (NOB); Nitrification; AMMONIA OXIDIZING BACTERIA; WASTE-WATER; MOLECULAR TECHNIQUES; PERFORMANCE; DIVERSITY; SEQUENCE; NITROGEN; REMOVAL; GENES;
D O I：
10.1016/j.biortech.2013.09.050
中图分类号：
S2 [农业工程];
学科分类号：
0828 ;
摘要：
This study investigated the nitrifying bacterial community in membrane bioreactor (MBR) at short solids retention times (SRTs) of 3, 5 and 10 days. The denaturing gradient gel electrophoresis results showed that different types of ammonia-oxidizing bacteria (AOB) can survive at different operating conditions. The diversity of AOB increased as the SRT increased. The real-time PCR results showed that the amoA gene concentrations were similar when MBRs were stabilized, and it can be a good indicator of stabilized nitrification. The results of clone library indicated that Nitrosomonas was the dominant group of AOB in three reactors. The microarray results showed that Nitrospira was the dominant group of nitrite-oxidizing bacteria (NOB) in the system. All groups of AOB and NOB except Nitrosolobus and Nitro coccus were found in MBR, indicated that the nitrifying bacterial community structure was more complicated. The combination of some molecular tools can provide more information of microbial communities. (C) 2013 Elsevier Ltd. All rights reserved.
引用
收藏
页码：200 / 207
页数：8
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