Diagnostic Utility of the B-cell Lineage Markers CD20, CD79a, PAX5. and CD19 in Paraffin-embedded Tissues From Lymphoid Neoplasms

被引：38

作者：

Adams, Heiner ^{[1
]}

Liebisch, Peter ^{[2
]}

Schmid, Patrik ^{[1
]}

Dirnhofer, Stephan ^{[1
]}

Tzankov, Alexandar ^{[1
]}

机构：

[1] Univ Basel Hosp, Inst Pathol, CH-4003 Basel, Switzerland

[2] Univ Hosp Ulm, Dept Internal Med 3, Ulm, Germany

来源：

APPLIED IMMUNOHISTOCHEMISTRY & MOLECULAR MORPHOLOGY | 2009年 / 17卷 / 02期

关键词：

CD20; CD79a; CD19; PAX5; lymphoma; leukemia; tissue microarrays; HODGKIN/REED-STERNBERG CELLS; ACUTE LYMPHOBLASTIC-LEUKEMIA; EXPRESSION; RECEPTOR; COMPLEX; BSAP;

D O I：

10.1097/PAI.0b013e3181845ef4

中图分类号：

R602 [外科病理学、解剖学]; R32 [人体形态学];

学科分类号：

100101 ;

摘要：

The specificity and sensitivity of CD 19, CD20, CD79a, and PAX5 for detection of B-cell lineage lymphoma/leukemia derivation was determined on tissue microarrays containing 148 Hodgkin lymphomas, 358 B-cell and 16 T-cell lymphomas, 50 myelomas, and 69 acute leukemias. In Mature lymphoid neoplasms, receiver-operating characteristic curve analysis showed CD20 to be the most sensitive, and CD20 and CD79a the most specific markers for B-lineage derivation. CD19 had the weakest specificity, because it was expressed in 3 T-cell lymphomas, but its sensitivity was better than CD79a. In Hodgkin lymphoma cases, the presence of B-cell markers in Hodgkin and Reed-Sternberg cells decreased in the following order: PAX5 > CD20 > CD79a > CD19. CID 19 and PAX5 were not detectable in myelomas. In acute leukemia, CD20 turned to be the most specific, and PAX5 and CD19 the most sensitive markers for B-lineage derivation. In conclusion, all optimal B-cell lineage panel for daily routine on paraffin-embedded tissues should consist of CD20 and CD79a, and eventually, PAX5 for nature lymphoid neoplasms and PAX5 and CD19, and eventually, CD20 in (acute) precursor cell leukemias, because they cover most of the sensitivity and specificity needed.

引用

页码：96 / 101

页数：6

共 35 条

[1]

[Anonymous], 2001, Pathology and Genetics: Tumours of Haematopoietic and Lymphoid Tissues

[2] Chromosomal instability in gastric mucosa-associated lymphoid tissue lymphomas: a fluorescent in situ hybridization study using a tissue microarray approach [J].

Bernasconi, Barbara ;

Karamitopolou-Diamantiis, Eva ;

Tornillo, Luigi ;

Lugli, Alessandro ;

Di Vizio, Dolores ;

Dirnhofer, Stephan ;

Wengmann, Stephan ;

Glatz-Krieger, Katharyna ;

Fend, Falko ;

Capella, Carlo ;

Insabato, Luigi ;

Terracciano, Luigi M. .

HUMAN PATHOLOGY, 2008, 39 (04) :536-542

[3]

Berthold D, 2004, SWISS MED WKLY, V134, P472

[4] Anaplastic large cell lymphomas lack the expression of T-cell receptor molecules or molecules of proximal T-cell receptor signaling [J].

Bonzheim, I ;

Geissinger, E ;

Roth, S ;

Zettl, A ;

Marx, A ;

Rosenwald, A ;

Müller-Hermelink, HK ;

Rüdiger, T .

BLOOD, 2004, 104 (10) :3358-3360

[5] A novel PAX5-ELN fusion protein identified in B-cell acute lymphoblastic leukemia acts as a dominant negative on wild-type PAX5 [J].

Bousquet, Marina ;

Broccardo, Cyril ;

Quelen, Cathy ;

Meggetto, Fabienne ;

Kuhlein, Emilienne ;

Delsol, Georges ;

Dastugue, Nicole ;

Brousset, Pierre .

BLOOD, 2007, 109 (08) :3417-3423

[6]

BRADBURY LE, 1992, J IMMUNOL, V149, P2841

[7]

DELSOL G, 1993, AM J PATHOL, V142, P1729

[8] Levels of expression of CD19 and CD20 in chronic B cell leukaemias [J].

Ginaldi, L ;

De Martinis, M ;

Matutes, E ;

Farahat, N ;

Morilla, R ;

Catovsky, D .

JOURNAL OF CLINICAL PATHOLOGY, 1998, 51 (05) :364-369

[9]

HARADA H, 1993, BLOOD, V81, P2658

[10] Loss of B cell identity correlates with loss of B cell-specific transcription factors in Hodgkin/Reed-Sternberg cells of classical Hodgkin lymphoma [J].

Hertel, CB ;

Zhou, XG ;

Hamilton-Dutoit, SJ ;

Junker, S .

ONCOGENE, 2002, 21 (32) :4908-4920

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