Proteomics Profiling Reveals Carbohydrate Metabolic Enzymes and 14-3-3 Proteins Play Important Roles for Starch Accumulation during Cassava Root Tuberization

被引：52

作者：

Wang, Xuchu ^{[1
,2
]}

Chang, Lili ^{[1
,2
]}

Tong, Zheng ^{[1
]}

Wang, Dongyang ^{[1
,2
]}

Yin, Qi ^{[1
,2
]}

Wang, Dan ^{[1
]}

Jin, Xiang ^{[1
]}

Yang, Qian ^{[1
]}

Wang, Liming ^{[1
]}

Sun, Yong ^{[1
]}

Huang, Qixing ^{[1
]}

Guo, Anping ^{[1
]}

Peng, Ming ^{[1
,2
]}

机构：

[1] Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Key Lab Biol & Genet Resources Trop Crops, Haikou 571101, Hainan, Peoples R China

[2] Hainan Univ, Coll Agr, Haikou 570228, Hainan, Peoples R China

来源：

SCIENTIFIC REPORTS | 2016年 / 6卷

关键词：

MEMBRANE H+-ATPASE; STORAGE ROOT; BIOSYNTHESIS; EXPRESSION; GENOME; IDENTIFICATION; LEAVES; SPECIFICITY; PATHWAY; GENES;

D O I：

10.1038/srep19643

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Cassava is one of the most important root crops as a reliable source of food and carbohydrates. Carbohydrate metabolism and starch accumulation in cassava storage root is a cascade process that includes large amounts of proteins and cofactors. Here, comparative proteomics were conducted in cassava root at nine developmental stages. A total of 154 identified proteins were found to be differentially expressed during starch accumulation and root tuberization. Many enzymes involved in starch and sucrose metabolism were significantly up-regulated, and functional classification of the differentially expressed proteins demonstrated that the majority were binding-related enzymes. Many proteins were took part in carbohydrate metabolism to produce energy. Among them, three 14-3-3 isoforms were induced to be clearly phosphorylated during storage root enlargement. Overexpression of a cassava 14-3-3 gene in Arabidopsis thaliana confirmed that the older leaves of these transgenic plants contained higher sugar and starch contents than the wild-type leaves. The 14-3-3 proteins and their binding enzymes may play important roles in carbohydrate metabolism and starch accumulation during cassava root tuberization. These results not only deepened our understanding of the tuberous root proteome, but also uncovered new insights into carbohydrate metabolism and starch accumulation during cassava root enlargement.

引用

页数：15

共 64 条

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