An angiotensin-converting enzyme-inhibitory metabolite with partial structure of microginin in a cyanobacterium Anabaena fertilissima CCC597, producing fibrinolytic protease

被引：7

作者：

Bagchi, Suvendra Nath ^{[1
]}

Sondhia, Shobha ^{[2
]}

Agrawal, Manish Kumar ^{[3
]}

Banerjee, Sonali ^{[1
]}

机构：

[1] Rani Durgavati Univ, Dept Biol Sci, Jabalpur 482001, India

[2] Directorate Weed Sci Res, Dept Chem, Jabalpur 482004, India

[3] Excellent Bio Res Solut Pvt Ltd, 1370 Napier Town,Home Sci Coll Rd, Jabalpur 482001, India

来源：

JOURNAL OF APPLIED PHYCOLOGY | 2016年 / 28卷 / 01期

关键词：

Anabaena fertilissima; Antihypertensive; Cyanobacterial peptides; LC-MS/MS; Microginin;

D O I：

10.1007/s10811-015-0541-5

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Whole cell matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) of Anabaena fertilissima CCC597 revealed the presence of a microginin (M (r) = 565 Da), which was purified using ODS C18 solid-phase extraction followed by quaternary methyl ammonium (QMA)-ion exchange and reverse-phase liquid chromatography. Its partial structure was deduced upon LC-MS/MS as beta-amino-alpha-hydroxy-decanoic acid-Ala-MeLeu-Tyr. Microginin-565 inhibited rabbit angiotensin-converting enzyme activity at an apparent IC50 = 9.8 mu M.

引用

页码：177 / 180

页数：4

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