Rapid detection of heterotrophic growth of Haematococcus pluvialis using indirect conductimetry

被引:10
作者
Gong, XD [1 ]
Chen, F [1 ]
机构
[1] UNIV HONG KONG,DEPT BOT,HONG KONG,HONG KONG
关键词
D O I
10.1023/A:1018489611688
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
An indirect conductimetric method was developed for the rapid detection of heterotrophic growth of Haematococcus pluvialis. Selective HKU medium was superior to Whitley Impedance Broth. Acetate was better than glucose not only for the biomass production (30,200 cells ml(-1) on 1 g acetate l(-1) vs. 14,800 cells ml(-1) on 1 g glucose l(-1)) but also for the time needed to detect significant growth (10.9 h on 1 g acetate l(-1) vs. 36.9 h on 1 g glucose l(-1)). The largest maximum conductance change of -1455 mu S and the shortest detection time of 10.9 h were found in HKU medium containing 1 g acetate l(-1), and the cell concentration increased by 5.5 times after 4 days of cultivation.
引用
收藏
页码:841 / 844
页数:4
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