RNA-Seq analysis of differentially expressed genes relevant to mismatch repair in aging hematopoietic stem-progenitor cells

被引：5
作者：
Lian, Xiaolan [1 ,4 ]
Dong, Yongpin [2 ]
Zhao, Mingyi [3 ]
Liang, Yajie [4 ]
Jiang, Weiwei [2 ]
Li, Wenfang [2 ]
Zhang, Lina [1 ]
机构：
[1] Shanghai Univ Tradit Chinese Med, Inst Basic Med, Dept Biochem, 1200 CaiLun Ave, Shanghai 201203, Peoples R China
[2] Second Mil Med Univ, Shanghai Changzheng Hosp, Dept Emergency & Crit Care Med, Shanghai, Peoples R China
[3] Cent South Univ, Xiangya Hosp 3, Dept Pediat, Changsha, Hunan, Peoples R China
[4] Fujian Med Univ, Fujian Inst Hematol, Fujian Prov Key Lab Hematol, Union Hosp, Fuzhou, Fujian, Peoples R China
来源：
JOURNAL OF CELLULAR BIOCHEMISTRY | 2019年 / 120卷 / 07期
基金：
中国国家自然科学基金;
关键词：
aging; hematopoietic stem and progenitor cells; mismatch repair; RNA-Seq; REPLICATION PROTEIN-A; EXONUCLEASE; DNA-REPAIR; RPA;
D O I：
10.1002/jcb.28417
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We used RNA-sequencing (RNA-Seq) technology and an old hematopoietic stem and progenitor cells (HSPCs) model in vitro to analyze differential expressions of mismatch repair (MMR)-related genes in aged HSPCs, so as to explore the mechanism of DNA MMR injury inhematopoietic stem cells (HSC) aging. In this study, by combining RNA-seq data and National Center for Biotechnology Information database, we focus on six widely reported MMR genes MSH2, MSH3, MSH6, MLH1, PMS1, PMS2, and five MMR genes with closer ties to HSC aging Pcna, Exo1, Rpa1, Rpa2, and Rpa3 according to the genes functional classification and the related signaling pathway. It is concluded that MMR is closely related to HSC aging. This study provides experimental evidence for future researching MMR in HSC aging.
引用
收藏
页码：11401 / 11408
页数：8
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