Novel DNA methylation targets in oral rinse samples predict survival of patients with oral squamous cell carcinoma

被引：22

作者：

Langevin, Scott M. ^{[1
]}

Butler, Rondi A. ^{[2
]}

Eliot, Melissa ^{[2
]}

Pawlita, Michael ^{[3
]}

Maccani, Jennifer Z. J. ^{[4
,5
]}

McClean, Michael D. ^{[6
]}

Kelsey, Karl T. ^{[1
,7
]}

机构：

[1] Univ Cincinnati, Coll Med, Dept Environm Hlth, Cincinnati, OH 45267 USA

[2] Brown Univ, Dept Epidemiol, Providence, RI 02912 USA

[3] German Canc Res Ctr, Res Program Infect & Canc, Heidelberg, Germany

[4] Brown Univ, Dept Psychiat & Human Behav, Alpert Med Sch, Providence, RI 02912 USA

[5] Miriam Hosp, Ctr Behav & Prevent Med, Providence, RI 02906 USA

[6] Boston Univ, Sch Publ Hlth, Dept Environm Hlth, Boston, MA USA

[7] Brown Univ, Dept Pathol & Lab Med, Providence, RI 02912 USA

来源：

ORAL ONCOLOGY | 2014年 / 50卷 / 11期

关键词：

Oral cancer; Prognosis; Mouthwash; Epigenomics; GABBR1; GENE PROMOTER HYPERMETHYLATION; CPG ISLAND SHORES; SALIVARY RINSES; NECK-CANCER; BODY-FLUIDS; HEAD; TUMOR; OPCML; IDENTIFICATION; EXPRESSION;

D O I：

10.1016/j.oraloncology.2014.08.015

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Objectives: The objective of this study was to identify novel survival-associated biomarkers in oral rinse samples collected from patients with oral squamous cell carcinoma (OSCC). Materials and methods: We screened for putative survival-associated markers using publicly available methylation array data from 88 OSCC tumors. Cox models were then fit to methylation array data restricted to these putative loci in oral rinse samples of 82 OSCC patients from greater Boston. Pyrosequencing assays were designed for each locus that replicated in the oral rinse samples and applied to a validation set of oral rinse samples from another 61 OSCC patients. Results: We identified 7 survival-associated methylation markers in oral rinse samples from OSCC patients, and have validated one, located in the body of GABBR1, by pyrosequencing. Conclusion: The 7 CpG loci identified through this study represent novel prognostic biomarkers for patients with OSCC that can be detected using a non-invasive oral rinse collection technique. (C) 2014 Elsevier Ltd. All rights reserved.

引用

页码：1072 / 1080

页数：9

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