Characterization of a Novel Plasmid-Borne Thiopeptide Gene Cluster in Staphylococcus epidermidis Strain 115

被引:39
作者
Bennallack, Philip R. [1 ]
Burt, Scott R. [2 ]
Heder, Michael J. [1 ]
Robison, Richard A. [1 ]
Griffitts, Joel S. [1 ]
机构
[1] Brigham Young Univ, Dept Mol Biol & Microbiol, Provo, UT 84602 USA
[2] Brigham Young Univ, Dept Chem & Biochem, Provo, UT 84602 USA
关键词
RIBOSOMAL GTPASE CENTER; ANTIBIOTIC MICROCOCCIN; PROTEIN-SYNTHESIS; POSTTRANSLATIONAL MODIFICATIONS; BACTERIAL INTERFERENCE; PLASMODIUM-FALCIPARUM; BACILLUS-CEREUS; CANCER-CELLS; THIOSTREPTON; PEPTIDE;
D O I
10.1128/JB.02243-14
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Thiopeptides are small (12- to 17-amino-acid), heavily modified peptides of bacterial origin. This antibiotic family, with more than 100 known members, is characterized by the presence of sulfur-containing heterocyclic rings and dehydrated residues within a macrocyclic peptide structure. Thiopeptides, including micrococcin P1, have garnered significant attention in recent years for their potent antimicrobial activity against bacteria, fungi, and even protozoa. Micrococcin P1 is known to target the ribosome; however, like those of other thiopeptides, its biosynthesis and mechanisms of self-immunity are poorly characterized. We have discovered an isolate of Staphylococcus epidermidis harboring the genes for thiopeptide production and self-protection on a 24-kb plasmid. Here we report the characterization of this plasmid, identify the antimicrobial peptide that it encodes, and provide evidence of a target replacement-mediated mechanism of self-immunity.
引用
收藏
页码:4344 / 4350
页数:7
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