Detection of Mycoplasma canadense and Mycoplasma califomicum in dairy cattle from Argentina

被引：4
作者：
Tamiozzo, Pablo J. [1 ,2 ]
Estanguet, Abel A. [1 ]
Maito, Julia [3 ]
Tirante, Liliana [3 ]
Pol, Martin [3 ]
Giraudo, Jose A. [1 ]
机构：
[1] Univ Nacl Rio Cuarto, Fac Agron & Vet, Dept Patol Anim, Cordoba, Argentina
[2] Consejo Nacl Invest Cient & Tecn, Consejo Nacl Invest Cient & Tecn, RA-1033 Buenos Aires, DF, Argentina
[3] Lab Lactodiagnost Soc Responsabilidad Ltd SRL, Buenos Aires, DF, Argentina
来源：
REVISTA ARGENTINA DE MICROBIOLOGIA | 2014年 / 46卷 / 02期
关键词：
Dairy cattle; Mycoplasma bovis; Mycoplasma canadense; Mycoplasma californicum; ITS; 16S-23S rRNA; IDENTIFICATION; MOLLICUTES; SPACER; BOVIS; PCR;
D O I：
10.1016/S0325-7541(14)70059-8
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Different species of Mycoplasma can affect bovine cattle, causing several diseases. PCR sequencing and further analysis of the 16S-23S rRNA ITS region have shown a significant interspecies variability among Mollicutes. Sixteen suspected isolates of Mycoplasma spp. obtained from milk samples from dairy herds were amplified (165-23S rRNA ITS region). Fourteen out of those 16 suspected Mycoplasma spp. isolates were PCR-positive. To confirm the identity of Mycoplasma bovis, these 14 isolates were tested by another species-specific PCR. Seven of the isolates rendered a positive result. The products of 16S-23S rRNA ITS PCR from one isolate that was identified as M. bovis and from two other isolates, identified as non- M. bovis were randomly selected, sequenced and analyzed. The three sequences (A, B and C) showed 100% similarity with M. bovis, Mycoplasma canadense and Mycoplasma californicum respectively. (C) 2013 Asociacion Argentina de Microbiologia. Published by Elsevier Espana, S.L. All rights reserved.
引用
收藏
页码：119 / 121
页数：3
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