Salicornia bigelovii Torr Attenuates Neuro-Inflammatory Responses in Lipopolysaccharide-Induced BV-2 Microglia by Regulation of NF-kappa B Signaling

被引:5
作者
Kang, Hyun [1 ,2 ]
Koppula, Sushruta [1 ]
Park, Tae-Kyu [1 ]
机构
[1] Konkuk Univ, Coll Biomed & Hlth Sci, Res Inst Biomed & Hlth Sci, Dept Biotechnol, Chungju 380701, South Korea
[2] Konkuk Univ, Business Incubat Ctr, KuGen Healthcare Inst, Chungju 380701, South Korea
关键词
Salicornia bigelovii; Anti-oxidant; lipopolysaccharide; Neuroinflammation; Microglia; Cyclooxygenase; iNOS; NF-kappa B; NEURODEGENERATION;
D O I
10.4314/tjpr.v12i6.6
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Purpose: To investigate the anti-oxidant and anti-neuroinflammatory effects of Salicornia bigelovii extract (SBE) in lipopolysaccharide (LPS)-stimulated BV-2 microglial cells. Methods: Anti-oxidant activity was measured using 1, 1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging assay. Cell viability was evaluated using 3-(4, 5-dimethylthiazol-2-yl)-2, 5 -diphenyltetrazolium bromide (MTT) assay. BV- microglial cells were stimulated with LPS to study the protein expression and production of inflammatory mediators, determined by Western blot analysis. Results: SBE significantly inhibited the DPPH-generated free radicals showing maximum inhibition at 40 mu g/mL (p < 0.001). SBE alone did not exhibit any signs of cytotoxicity to BV-2 cells up to 200 mu g/mL concentration. The LPS-induced increase in the production of nitric oxide was concentration-dependently suppressed by SBE (p < 0.05 for 10 mu g/mL, p < 0.01 at 20 mu g/mL and p < 0.001 at 40 mu g/mL, respectively). SBE also inhibited the LPS-induced increase in inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expressions. Further, the production of proinflammatory cytokines such as tumor necrosis factor-alpha and interleukin-6 by LPS-stimulation in BV-2 cells was inhibited by SBE pretreatment. Mechanistic study revealed that SBE acts by regulation of nuclear factor kappa-B signaling pathway in LPS-stimulated BV-2 microglial cells. Conclusion: This study revealed for the first time that SBE possesses anti-oxidant and anti-neuroinflammatory effects and can be developed as a potential therapeutic target in ameliorating microglia-mediated neuroinflammation.
引用
收藏
页码:897 / 903
页数:7
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