o-Nitroanilines as major metabolic products of anti-Trypanosoma cruzi 5-phenylethenylbenzofuroxans in microsomal and cytosolic fractions of rat hepatocytes and in whole parasitic cells

被引：17
作者：
Boiani, M.
Merlino, A.
Gerpe, A.
Porcal, W.
Croce, F.
Depaula, S. [2 ]
Rodriguez, M. A. [2 ]
Cerecetto, H. [1 ]
Gonzalez, M.
机构：
[1] Univ Republica, Fac Ciencias, Lab Quim Organ, Fac Quim,Dept Quim Organ, Montevideo 11400, Uruguay
[2] Univ Republ, Fac Quim, Area Anal Quim, Pando, Uruguay
来源：
XENOBIOTICA | 2009年 / 39卷 / 03期
关键词：
Chagas disease; benzofuroxan; metabolism; rat hepatocytes microsomes; rat hepatocytes cytosolic fraction; Trypanosoma cruzi; POTENTIAL ANTITRYPANOSOMAL DRUGS; N-OXIDE DERIVATIVES; CHAGAS-DISEASE; IN-VITRO; IDENTIFICATION; BENZOFUROXANS; GUIDELINES; INHIBITORS; REDUCTION; GROWTH;
D O I：
10.1080/00498250802691535
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
The metabolism of six anti-Trypanosoma cruzi 5-phenylethenylbenzofuroxans (PhEBfx) was studied in vitro using rat hepatic microsomal and cytosolic fractions as a mammalian model and whole cells of T. cruzi as a parasitic model. Some of the expected metabolites were synthesized to provide authentic chromatographic standards. The metabolites were identified using high-performance liquid chromatography (HPLC) in comparison with the authentic standards and their proportions were determined. Their structures were confirmed using mass spectrometry (MS) and nuclear magnetic resonance (NMR) spectroscopy. The behaviour of the six PhEBfx in the three different systems was similar. The main metabolites, formed by reductive processes, were the corresponding o-nitroanilines. Two of the test compounds were studied for extended time periods in the rat liver preparations and their terminal metabolites were identified as o-phenylendiamine derivatives.
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收藏
页码：236 / 248
页数：13
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