Quartz crystal microbalance based biosensor for rapid and sensitive detection of maize chlorotic mottle virus

被引：32

作者：

Huang, Xin ^{[1
]}

Xu, Jianmin ^{[1
]}

Ji, Hai-Feng ^{[2
]}

Li, Guifen ^{[1
]}

Chen, Hongjun ^{[1
]}

机构：

[1] Chinese Acad Inspect & Quarantine, Inst Plant Quarantine, Beijing 100029, Peoples R China

[2] Drexel Univ, Dept Chem, Philadelphia, PA 19010 USA

来源：

ANALYTICAL METHODS | 2014年 / 6卷 / 13期

关键词：

SELF-ASSEMBLED MONOLAYERS; POLYELECTROLYTE MULTILAYERS; PROTEIN MOLECULES; RESONATOR; IMMOBILIZATION; LIQUID; CHINA;

D O I：

10.1039/c4ay00292j

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

In this work, we report a biosensor based on a quartz crystal microbalance ( QCM) for the selective and sensitive detection of maize chlorotic mottle virus (MCMV). A mixture of 10 : 1 3-mercaptopropanoic acid and 11-mercaptoundecanoic acid was applied on gold surfaces of QCM crystals to form a selfassembled monolayer, and an anti-MCMV antibody was crosslinked on the surface for specific recognition of MCMV. The frequency change of the QCM crystal is a function of the concentration of MCMV with a response range from 250 ng mL(-1) to 10 mg mL(-1). The standard curve is R2 0.997, indicating a high reproducibility in MCMV detection. The detection limit is approximately 250 ng mL(-1), which is similar to that of the existing enzyme-linked immunosorbent assay (ELISA) method. The QCM sensor showed highly specific and sensitive recognition for both purified MCMV and crude extracts from corn leaf samples.

引用

页码：4530 / 4536

页数：7

共 30 条

[1] HOST RANGE AND SEED-TRANSMISSION STUDIES OF MAIZE CHLOROTIC MOTTLE VIRUS IN GRASSES AND CORN
BOCKELMAN, DL
CLAFLIN, LE
UYEMOTO, JK
[J]. PLANT DISEASE, 1982, 66 (03) : 216 - 218
[2] Castillo J., 1974, Fitopatologia, V9, P79
[3] Enhanced sensitivity of self-assembled-monolayer-based SPR immunosensor for detection of benzaldehyde using a single-step multi-sandwich immunoassay
Gobi, K. Vengatajalabathy
Matsumoto, Kiyoshi
Toko, Kiyoshi
Ikezaki, Hidekazu
Miura, Norio
[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2007, 387 (08) : 2727 - 2735
[4] HaiYan G., 2010, ACTA PHYTOPATHOL SIN, V40, P426
[5] Microcantilever biosensors based on conformational change of proteins
Ji, Hai-Feng
Gao, Hongyan
Buchapudi, Koutilya R.
Yang, Xin
Xu, Xiaohe
Schulte, Marvin K.
[J]. ANALYST, 2008, 133 (04) : 434 - 443
[6] FREQUENCY OF A QUARTZ MICROBALANCE IN CONTACT WITH LIQUID
KANAZAWA, KK
GORDON, JG
[J]. ANALYTICAL CHEMISTRY, 1985, 57 (08) : 1770 - 1771
[7] Electronically monitoring biological interactions with carbon nanotube field-effect transistors
Kauffman, Douglas R.
Star, Alexander
[J]. CHEMICAL SOCIETY REVIEWS, 2008, 37 (06) : 1197 - 1206
[8] Characterization of DNA immobilization and subsequent hybridization on a 2D arrangement of streptavidin on a biotin-modified lipid bilayer supported on SiO2
Larsson, C
Rodahl, M
Höök, F
[J]. ANALYTICAL CHEMISTRY, 2003, 75 (19) : 5080 - 5087
[9] A combined nanoplasmonic and electrodeless quartz crystal microbalance setup
Larsson, Elin M.
Edvardsson, Malin E. M.
Langhammer, Christoph
Zoric, Igor
Kasemo, Bengt
[J]. REVIEW OF SCIENTIFIC INSTRUMENTS, 2009, 80 (12)
[10] CHARACTERIZATION OF MAIZE CHLOROTIC MOTTLE VIRUS
LOMMEL, SA
KENDALL, TL
SIU, NF
NUTTER, RC
[J]. PHYTOPATHOLOGY, 1991, 81 (08) : 819 - 823

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