Integrated analysis of microRNA and mRNA expression profiles in abdominal adipose tissues in chickens

被引：80

作者：

Huang, H. Y. ^{[1
,2
]}

Liu, R. R. ^{[1
]}

Zhao, G. P. ^{[1
]}

Li, Q. H. ^{[1
]}

Zheng, M. Q. ^{[1
]}

Zhang, J. J. ^{[1
]}

Li, S. F. ^{[2
]}

Liang, Z. ^{[2
]}

Wen, J. ^{[1
]}

机构：

[1] Chinese Acad Agr Sci, Inst Anim Sci, Beijing 100193, Peoples R China

[2] Chinese Acad Agr Sci, Inst Poultry Sci, Yangzhou 225125, Peoples R China

来源：

SCIENTIFIC REPORTS | 2015年 / 5卷

关键词：

FATTY-ACID; ADIPOCYTE DIFFERENTIATION; IN-VIVO; IDENTIFICATION; TRAITS; GENES; FADS2; DESATURASE; OXIDATION; ABUNDANCE;

D O I：

10.1038/srep16132

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Excessive fat accretion is a crucial problem during broiler production. Abdominal fat weight (AbFW) and abdominal fat percentage (AbFP) are major phenotypic indices of fat traits. The present study used F2 females derived from a cross between Beijing-You and Cobb-Vantress chickens. Cohorts with extreme AbFP and AbFW phenotypes were chosen to construct high- and low-abdominal fat libraries (HAbF and LAbF, respectively) to investigate the expression profiles by RNA-sequencing and microRNA (miRNA)-sequencing. Compared with the LAbF library, 62 differentially expressed miRNAs (DEMs) and 303 differentially expressed genes (DEGs) were identified in the HAbF birds. Integrated analysis of DEMs and DEGs showed that a total of 106 DEGs were identified as target genes for the 62 DEMs. These genes were designated as intersection genes, and 11 of these genes are involved in lipid metabolism pathways. The miRNA gga-miR-19b-3p accelerated the proliferation of preadipocytes, as well as adipocyte differentiation, by down-regulating ACSL1. These findings suggest that some strong candidate miRNAs and genes, important in relation to abdominal adipose deposition, were identified by the integrated analysis of DEMs and DEGs. These findings add to our current understanding of the molecular genetic controls underlying abdominal adipose accumulation in chickens.

引用

页数：14

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