A sensitive colorimetric high-throughput screening method for lipase synthetic activity assay

被引：21
|
作者：
Zheng, Jianyong [1 ]
Fu, Xianfeng [1 ]
Ying, Xiangxian [1 ]
Zhang, Yinjun [1 ]
Wang, Zhao [1 ]
机构：
[1] Zhejiang Univ Technol, Coll Biol & Environm Engn, Hangzhou 310032, Zhejiang, Peoples R China
来源：
ANALYTICAL BIOCHEMISTRY | 2014年 / 452卷
基金：
国家高技术研究发展计划(863计划);
关键词：
Lipase; High-throughput screening; Synthetic activity; MBTH; Derivatization; NITROPHENYL PALMITATE ASSAY; TRIAD FORMS; TRANSESTERIFICATION;
D O I：
10.1016/j.ab.2014.02.001
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A sensitive and practical high-throughput screening method for assaying lipase synthetic activity is described. Lipase-catalyzed transesterification between vinyl acetate and n-butanol in n-hexane was chosen as a model reaction. The released acetaldehyde was determined by the colorimetric method using 3-methyl-2-benzothialinone (MBTH) derivatization. In comparison with other methods, the major advantages of this process include high sensitivity, simple detection, inexpensive reagents, and low requirements for instruments. (C) 2014 Elsevier Inc. All rights reserved.
引用
收藏
页码：13 / 15
页数：3
相关论文
共 50 条
  • [31] High-throughput screening: advances in assay technologies
    Sittampalam, GS
    Kahl, SD
    Janzen, WP
    CURRENT OPINION IN CHEMICAL BIOLOGY, 1997, 1 (03) : 384 - 391
  • [32] High-throughput screening - Free your assay
    Kirkpatrick, P
    NATURE REVIEWS DRUG DISCOVERY, 2004, 3 (07) : 553 - 553
  • [33] Phospholipid/Polydiacetylene Vesicle-Based Colorimetric Assay for High-Throughput Screening of Bacteriocins and Halocins
    Manoj Kumar Yadav
    Vijay Kumar
    Bijender Singh
    Santosh Kumar Tiwari
    Applied Biochemistry and Biotechnology, 2017, 182 : 142 - 154
  • [34] Phospholipid/Polydiacetylene Vesicle-Based Colorimetric Assay for High-Throughput Screening of Bacteriocins and Halocins
    Yadav, Manoj Kumar
    Kumar, Vijay
    Singh, Bijender
    Tiwari, Santosh Kumar
    APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2017, 182 (01) : 142 - 154
  • [35] Continuous Colorimetric Assay That Enables High-Throughput Screening of N-Acetylamino Acid Racemases
    Sanchez-Carron, Guiomar
    Fleming, Toni
    Holt-Tiffin, Karen E.
    Campopiano, Dominic J.
    ANALYTICAL CHEMISTRY, 2015, 87 (07) : 3923 - 3928
  • [36] High-throughput colorimetric method for the parallel assay of glyoxylic acid and ammonium in a single extract
    Brautigam, Andrea
    Gagneul, David
    Weber, Andreas P. M.
    ANALYTICAL BIOCHEMISTRY, 2007, 362 (01) : 151 - 153
  • [37] Development of a high-throughput colorimetric Zika virus infection assay
    Janis A. Müller
    Mirja Harms
    Axel Schubert
    Benjamin Mayer
    Stephanie Jansen
    Jean-Philippe Herbeuval
    Detlef Michel
    Thomas Mertens
    Olli Vapalahti
    Jonas Schmidt-Chanasit
    Jan Münch
    Medical Microbiology and Immunology, 2017, 206 : 175 - 185
  • [38] Development of a high-throughput colorimetric Zika virus infection assay
    Mueller, Janis A.
    Harms, Mirja
    Schubert, Axel
    Mayer, Benjamin
    Jansen, Stephanie
    Herbeuval, Jean-Philippe
    Michel, Detlef
    Mertens, Thomas
    Vapalahti, Olli
    Schmidt-Chanasit, Jonas
    Muench, Jan
    MEDICAL MICROBIOLOGY AND IMMUNOLOGY, 2017, 206 (02) : 175 - 185
  • [39] A fluorogenic histone deacetylase assay well suited for high-throughput activity screening
    Wegener, D
    Wirsching, F
    Riester, D
    Schwienhorst, A
    CHEMISTRY & BIOLOGY, 2003, 10 (01): : 61 - 68
  • [40] Development of an assay suitable for high-throughput screening to measure matrix metalloprotease activity
    Peppard, J
    Pharn, Q
    Clark, A
    Farley, D
    Sakane, Y
    Graves, R
    George, J
    Norey, C
    ASSAY AND DRUG DEVELOPMENT TECHNOLOGIES, 2003, 1 (03) : 425 - 433
12345