A sensitive colorimetric high-throughput screening method for lipase synthetic activity assay

被引：21
|
作者：
Zheng, Jianyong [1 ]
Fu, Xianfeng [1 ]
Ying, Xiangxian [1 ]
Zhang, Yinjun [1 ]
Wang, Zhao [1 ]
机构：
[1] Zhejiang Univ Technol, Coll Biol & Environm Engn, Hangzhou 310032, Zhejiang, Peoples R China
来源：
ANALYTICAL BIOCHEMISTRY | 2014年 / 452卷
基金：
国家高技术研究发展计划(863计划);
关键词：
Lipase; High-throughput screening; Synthetic activity; MBTH; Derivatization; NITROPHENYL PALMITATE ASSAY; TRIAD FORMS; TRANSESTERIFICATION;
D O I：
10.1016/j.ab.2014.02.001
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A sensitive and practical high-throughput screening method for assaying lipase synthetic activity is described. Lipase-catalyzed transesterification between vinyl acetate and n-butanol in n-hexane was chosen as a model reaction. The released acetaldehyde was determined by the colorimetric method using 3-methyl-2-benzothialinone (MBTH) derivatization. In comparison with other methods, the major advantages of this process include high sensitivity, simple detection, inexpensive reagents, and low requirements for instruments. (C) 2014 Elsevier Inc. All rights reserved.
引用
收藏
页码：13 / 15
页数：3
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