Development of PCR assay to identify Pseudomonas fluorescens and its biotype

被引：78

作者：

Scarpellini, M ^{[1
]}

Franzetti, L ^{[1
]}

Galli, A ^{[1
]}

机构：

[1] Dipartimento Sci & Tecnol Alimentari & Microbiol, Sez Microbiol Agraria Alimentare Ecol, I-20133 Milan, Italy

来源：

FEMS MICROBIOLOGY LETTERS | 2004年 / 236卷 / 02期

关键词：

Pseudomonas fluorescens; 16S rRNA; biotype; primer species-specific;

D O I：

10.1016/j.femsle.2004.05.043

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The paper provides a simple protocol that uses the polymerase chain reaction to amplify a specific portion of the 16S gene, allowing the recognition of Pseudomonas fluorescens from other group I Pseudomonas. The amplified DNA patterns of 16S rRNA and ITS1, from the restriction fragment length polymorphisms VspI, HaeIII and TaqI digestion, produced band patterns that distinguished the biotypes of Ps. fluorescens. In addition to distinguishing the biotypes C and 3 we used a phenotypical method for levan production. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：257 / 260

页数：4

共 14 条

[1] The oligonucleotide probe database [J].