The complete mitochondrial genome of the southern purple- spotted gudgeon Mogurnda adspersa ( Perciformes: Eleotridae) through pyrosequencing

被引:3
|
作者
Perini, Violeta da Rocha [1 ]
de Carvalho, Daniel Cardoso [2 ,3 ]
Beheregaray, Luciano Bellagamba [3 ]
Prosdocimi, Francisco [1 ]
机构
[1] Univ Fed Rio de Janeiro, Multidisciplinary Lab Data Anal, Inst Bioquim Med Leopoldo Meis, Rio De Janeiro, Brazil
[2] Pontificia Univ Catolica Minas Gerais, Conservat Genet Lab, Postgrad Studies Zool, PUC, Belo Horizonte, MG, Brazil
[3] Flinders Univ S Australia, Sch Biol Sci, Mol Ecol Lab, Adelaide, SA 5001, Australia
基金
澳大利亚研究理事会;
关键词
Fish; mitogenome; next-generation-sequencing; SEQUENCE; FISH; CONSERVATION; GENERATION; ANNOTATION; AUSTRALIA; DATABASE;
D O I
10.3109/19401736.2014.895995
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
The complete mitochondrial genome of the Critically Endangered southern purple-spotted gudgeon, Mogurnda adspersa, was determined for the first time using 1/8 of a 454 pyrosequencing plate. The mitogenome was assembled using the bioinformatic software MIRA. The M. adspersa genome organization was very similar to most vertebrates, being 16,523bp in length. It contained 13 protein-coding genes, 22 transfer RNA, 2 ribosomal RNA genes, and 1 non-coding control region. The current methodology was effective to assemble the whole mitogenome using 2008 mitochondrial reads representing 0.23% of total genomic reads produced and providing average mitogenome coverage of 17.4 reads per site. The whole mitogenome sequence provided here is deposited in NCBI with the accession number KJ130031 and may benefit systematics studies and conservation programs of M. adspersa.
引用
收藏
页码:380 / 382
页数:3
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