Association between CRP polymorphisms and the risk of ischemic stroke

Purpose: This study aimed to detect the potential association between the single nucleotide polymorphisms (SNPs) of rs1130864, rs3093059 and rs1205 in C-reactive protein (CRP) gene and the risk of ischemic stroke (IS). Methods: 100 diagnosed IS patients and 106 healthy people were recruited into the case and control groups respectively in this study. The genotypes distributions of the control groups in rs1130864, rs3093059 and rs1205 were detected by Hardy-Weinberg equilibrium (HWE). TaqMan fluorescence probe was adopted to conduct quantitative detection and high throughput fluorescent quantitation PCR instrument was used to distinguish genotypes. Haploview software was applied to perform linkage disequilibrium and haplotype analysis. Odds ratio (OR) with 95% confidence interval (CI) was calculated by chi-square test to assess the association of CRP SNPs and haplotypes with IS risk. Results: The genotypes frequencies of rs1130864, rs3093059 and rs1205 in control group were all consistent with HWE. No statistically significant difference was found in genotypes and alleles distributions of rs1130864, rs3093059 and rs1205 in CRP gene between two groups (P>0.05). The haplotype C-C-C could significantly increase the risk of IS (OR=1.74, 95% CI=1.04-2.93). Conclusion: The rs1130864, rs3093059 and rs1205 polymorphisms of CRP gene are not related to the occurrence of IS, while C-C-C haplotype may be a risk factor for IS.