Characterization of amino acid substitutions in KdpA, the K+-binding and -translocating subunit of the KdpFABC complex of Escherichia coli

被引：29

作者：

van der Laan, M ^{[1
]}

Gassel, M ^{[1
]}

Altendorf, K ^{[1
]}

机构：

[1] Univ Osnabruck, Fachbereich Biol Chem, Abt Mikrobiol, D-49069 Osnabruck, Germany

来源：

JOURNAL OF BACTERIOLOGY | 2002年 / 184卷 / 19期

关键词：

D O I：

10.1128/JB.184.19.5491-5494.2002

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

When grown under K+ limitation, Escherichia coli induces the K+-translocating KdpFABC complex. The stimulation of ATPase activity by NH4+ ions was shown for the first time. Substitutions in KdpA, which is responsible for K+ binding and translocation, revealed that enzyme complexes KdpA:G232A and KdpA:G232S have completely lost their cation selectivity.

引用

页码：5491 / 5494

页数：4

共 23 条

[1]

Altendorf K, 1998, ACTA PHYSIOL SCAND, V163, P137

[2]

ALTENDORF K, 1996, BIOMEMBR, V5, P403

[3] GENETIC-EVIDENCE FOR 2 SEQUENTIALLY OCCUPIED K+ BINDING-SITES IN THE KDP TRANSPORT ATPASE [J].

BUURMAN, ET ;

KIM, KT ;

EPSTEIN, W .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (12) :6678-6685

[4]

BUURMAN ET, 1991, ARCH MICROBIOL, V155, P391

[5] Substrate-binding clusters of the K+-transporting kdp ATPase of Escherichia coli investigated by amber suppression scanning mutagenesis [J].

Dorus, S ;

Mimura, H ;

Epstein, W .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (13) :9590-9598

[6] The structure of the potassium channel:: Molecular basis of K+ conduction and selectivity [J].

Doyle, DA ;

Cabral, JM ;

Pfuetzner, RA ;

Kuo, AL ;

Gulbis, JM ;

Cohen, SL ;

Chait, BT ;

MacKinnon, R .

SCIENCE, 1998, 280 (5360) :69-77

[7] Does the KdpA subunit from the high affinity K+-translocating P-type KDP-ATPase have a structure similar to that of K+ channels? [J].

Durell, SR ;

Bakker, EP ;

Guy, HR .

BIOPHYSICAL JOURNAL, 2000, 78 (01) :188-199

[8] Evolutionary relationship between K+ channels and symporters [J].

Durell, SR ;

Hao, YL ;

Nakamura, T ;

Bakker, EP ;

Guy, HR .

BIOPHYSICAL JOURNAL, 1999, 77 (02) :775-788

[9] The Kdp-ATPase of Escherichia coli mediates an ATP-Dependelb, K+-independent electrogenic partial reaction [J].

Fendler, K ;

Dröse, S ;

Epstein, W ;

Bamberg, E ;

Altendorf, K .

BIOCHEMISTRY, 1999, 38 (06) :1850-1856

[10]

GASSEL M, 1999, THESIS U OSNABRUCK O

← 1 2 3 →