MEST promotes bladder cancer cell proliferation, migration and invasion via STAT3/Twist-1-mediated EMT

被引：7

作者：

Zhao, Cheng ^{[1
]}

Hu, Xiheng ^{[1
]}

Tong, Shiyu ^{[1
]}

Mo, Miao ^{[1
]}

He, Wei ^{[1
]}

Wang, Long ^{[1
]}

Li, Yangle ^{[1
]}

机构：

[1] Cent South Univ, Xiangya Hosp, Dept Urol, 87 Xiangya Rd, Changsha 410008, Peoples R China

来源：

TRANSLATIONAL CANCER RESEARCH | 2020年 / 9卷 / 10期

关键词：

Mesoderm-specific transcript (MEST); epithelial-mesenchymal transition; STAT3/Twist-1; signalling; bladder cancer (BC); EPITHELIAL-MESENCHYMAL TRANSITION; GROWTH-FACTOR-II; IMPRINTED GENES; FREQUENT LOSS; EXPRESSION; PEG1/MEST; STAT3; RELAXATION;

D O I：

10.21037/tcr-20-1006

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Background: Mesoderm-specific transcript (MEST) has been demonstrated to be a proto-oncogene or anti-oncogene in various carcinomas. However, the role and mechanism of MEST in bladder cancer (BC) are still unknown. Here we aimed to explore the effect of MEST on malignant biological behaviour in BC and its potential mechanism. Methods: The expression of MEST in BC tissues and cells was detected by qRT-PCR methods. MEST depletion and overexpression cell lines were established in T24 and 5637 respectively. Then the effects of MEST on cell proliferation, migration, invasion and epithelial-mesenchymal transition (EMT) were investigated. Finally, the STAT3/Twist-1 signaling was verified. Results: MEST was elevated in BC tissues and cells lines, and its high expression was highly relevant to the clinicopathologic features of patients with BC and to poor prognosis in these patients. MEST depletion impeded cell proliferation, migration and invasion as well as epithelial-mesenchymal transition (EMT), while MEST overexpression promoted malignant biological behaviour in BC. Mechanistically, MEST upregulated p-STAT3 and Twist-1 expression, while treatment with a STAT3 inhibitor clearly attenuated the STAT3 activation and Twist-1 upregulation induced by MEST. Subsequently, rescue assays confirmed that inhibition of STAT3 signalling could remarkably relieve the oncogenic effects of MEST on malignant biological behaviour in BC. Conclusions: Our data confirmed that MEST exerts oncogenic functions in bladder cancer via STAT3/Twist-1 signalling and that MEST may represent a promising target in BC treatment.

引用

页码：6178 / 6188

页数：11

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