Autotetraploid tangor plant regeneration from in vitro Citrus somatic embryogenic callus treated with colchicine

被引：64

作者：

Wu, JH ^{[1
]}

Mooney, P ^{[1
]}

机构：

[1] Hort & Food Res Inst New Zealand Ltd, Kerikeri Res Ctr, Kerikeri, New Zealand

来源：

PLANT CELL TISSUE AND ORGAN CULTURE | 2002年 / 70卷 / 01期

关键词：

chromosome doubling; chromosome squash; flow cytometry; mixoploid; oryzalin; somatic embryogenesis; tangor (Citrus reticulata Blanco x C sinensis [L.] Osb.); transplantation;

D O I：

10.1023/A:1016029829649

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In vitro chromosome doubling of embryogenic callus lines of the Citrus cultivars `Umatilla' and `Dweet' tangors (Citrus reticulata BlancoxC. sinensis [L.] Osb.), `Caffin' clementine (C. clementina Hort. ex Tan.) and `Wheeny' grapefruit (C. paradisi Macf) was carried out in the presence of either 0.05 or 0.1% colchicine, or 0.01, 0.05 or 0.1% oryzalin. Embryogenic callus development was partly suppressed in the presence of colchicine, and completely suppressed by oryzalin at all concentrations tested. No plants were regenerated from any of the oryzalin treatments. Ploidy level of plants regenerated from the colchicine treatments was determined using flow cytometry and chromosome squashes. Three desirable non-chimeric, autotetraploid plants of the mono-embryonic cultivar `Umatilla' were produced using 0.05% colchicine and one from 0.1% colchicine. One mixoploid `Dweet' plant was produced using 0.1% colchicine.

引用

页码：99 / 104

页数：6

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