Variations in mercury and other trace elements contents in soil and in vine leaves from the Almad,n Hg-mining district

Vines (Vitis vinifera, L.) are a very important agricultural resource for Spain in general and for the Castilla-La Mancha region in particular, providing important productions of wines. Grapes and raisins are used for direct consumption too. In this work, we study analytical constraints regarding metallic trace elements uptake, focusing on Hg, from vines growing in the Almad,n mercury mining district, the world's largest producer of this element, inactive nowadays. The study started with the analysis of these metals in soils and sets of vines leaves from seven sites located at different distances from the Almad,n Hg mine. The samples of soils were dried at ambient temperature for 1 week. They were then sifted (< 2 mm) and were stored for subsequent analysis. The leaves were dried and the leaf blade and petiole were separated. About 2 g of each sample were hand milled and analyzed using the same fluorescence spectrometer. Total mercury in soils and vine leaves were determined using a Lumex RA-915+ device, an atomic absorption spectrometer with a pyrolysis unit (RP-91c). Results show significant correlations between soil and leaves contents for total and organic mercury (R = 0.934 and 0.984, respectively). Hg contents range in soil from 2,376 to 0.04 mg/kg in non-polluted places. For the organic fraction, the range varies between 197.49 and 3.15 mg/kg. Total Hg contents measured in leaves were from 5.14 mg/kg (close to dump zone of the mine) to 0.03 mg/kg in the proximity of Carrin de Calatrava, located some 100 km away from Almad,n. Mercury reaches maximum in the proximity of known sources of the element: the mining and/or metallurgical areas of Almad,n and Almadenejos. Soils from the study area contain normal contents in trace metals, and these are conditioned by the local geology or urban locations of the area. Trace metals contents in leaves do not show a relationship with soil contents, possibly due to the low bioavailability of these elements in the soils investigated. Mercury is, as expected for this area, an exception to this trend, with very high concentrations that reach maximum values in the proximity of the known sources of the element: the proximity of mining and/or metallurgical areas in Almad,n and Almadenejos.