Protective role of edaravone against neomycin-induced ototoxicity in zebrafish

被引：19

作者：

Choi, June ^{[1
]}

Chang, Jiwon ^{[1
]}

Jun, Hyung Jin ^{[1
]}

Im, Gi Jung ^{[1
]}

Chae, Sung Won ^{[1
]}

Lee, Seung Hoon ^{[1
]}

Kwon, Soon-Young ^{[1
]}

Jung, Hak Hyun ^{[1
]}

Chung, Ah-Young ^{[2
]}

Park, Hae-Chul ^{[2
]}

机构：

[1] Korea Univ, Coll Med, Ansan Hosp, Dept Otorhinolaryngol Head & Neck Surg, Ansan, South Korea

[2] Korea Univ, Grad Sch Med, Lab Neurodev Genet, Ansan, South Korea

来源：

JOURNAL OF APPLIED TOXICOLOGY | 2014年 / 34卷 / 05期

关键词：

zebrafish; hearing; ototoxicity; neomycin; edaravone; HAIR CELL LOSS; RADICAL SCAVENGER EDARAVONE; VIVO DRUG DISCOVERY; LATERAL-LINE; IN-VIVO; GENTAMICIN; DEATH; REVEALS; MCI-186; MODEL;

D O I：

10.1002/jat.2964

中图分类号：

R99 [毒物学（毒理学）];

学科分类号：

100405 ;

摘要：

Aminoglycosides such as neomycin are one of the most commonly prescribed types of antibiotics worldwide. However, these drugs appear to generate free radicals within the inner ear, which can result in permanent hearing loss. We evaluated the effects of edaravone, a neuroprotective agent, on neomycin-induced ototoxicity in transgenic zebrafish. The 5-day post fertilization (dpf) zebrafish larvae were exposed to 125 mu M neomycin and various concentrations of edaravone for 1 h. Hair cell survival was calculated as average numbers of the hair cells in the control group, which was not exposed to neomycin. Ultrastructural changes were evaluated using a scanning electron microscope (SEM) and transmission electron microscope (TEM). Edaravone protected against neomycin-induced hair cell loss in the neuromasts (1000 mu M: 11.6 +/- 1.1 cells, neomycin only: 5.5 +/- 0.5 cells; n = 10, P < 0.05) and decreased the TUNEL reaction for detecting apoptosis. In ultrastructural analysis, structures of mitochondria and hair cells within neuromasts were preserved in zebrafish exposed to 125 mu M neomycin and 1000 mu M edaravone for 1 h. Edaravone protected against neomycin-induced hair cell loss by preventing apoptosis. Copyright (c) 2013 John Wiley & Sons, Ltd.

引用

页码：554 / 561

页数：8

共 62 条

[1] The radical scavenger edaravone (3-methyl-1-phenyl-2-pyrazolin-5-one) reacts with a pterin derivative and produces a cytotoxic substance that induces intracellular reactive oxygen species generation and cell death [J].