Micropropagation of walnut trees (Juglans regia L) and response to arbuscular mycorrhizal inoculation

被引:28
作者
DolcetSanjuan, R [1 ]
Claveria, E [1 ]
Camprubi, A [1 ]
Estaun, V [1 ]
Calvet, C [1 ]
机构
[1] Inst Recerca & Tecnol Agroalimentaires IRTA, CTR CABRILS, DEPT GENET VEGETAL, Cabrils 08348, BARCELONA, SPAIN
来源
AGRONOMIE | 1996年 / 16卷 / 10期
关键词
walnut; micropropagation; rooting; acclimatization; arbuscular mycorrhiza;
D O I
10.1051/agro:19961008
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Juglans regia clones were micropropagated through the establishment of in vitro shoot-tip cultures and induction of adventitious roots. Shoot proliferation of juvenile J regia material was higher than with clones established from adult trees. Genotype and juvenility of the plant material were determinant factors of the in vitro rooting ability. Rooting of adult J regia clones was improved after rejuvenation by subsequent subcultures. The sucrose concentration in the root elongation medium affected the formation of secondary roots per rooted shoot and the acclimatization of plants. Mycorrhizal inoculation of micropropagated walnut trees improved post-acclimatization growth in greenhouse conditions. Inoculation with the arbuscular mycorrhizal fungi Glomus mosseae or G intraradices significantly improved plant survival when transferred to nursery.
引用
收藏
页码:639 / 645
页数:7
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