Bioimaging assessment and effect of skin wound healing using bone-marrow-derived mesenchymal stromal cells with the artificial dermis in diabetic rats

被引：25
作者：
Inoue, Hirokazu [1 ,2 ]
Murakami, Takashi [1 ]
Ajiki, Takashi [1 ,2 ]
Hara, Mayumi [1 ]
Hoshino, Yuichi [2 ]
Kobayashi, Eiji [1 ]
机构：
[1] Jichi Med Univ, Ctr Mol Med, Div Organ Replacement Res, Shimotsuke, Tochigi 3290498, Japan
[2] Jichi Med Univ, Dept Orthoped, Shimotsuke, Tochigi 3290498, Japan
来源：
JOURNAL OF BIOMEDICAL OPTICS | 2008年 / 13卷 / 06期
关键词：
wound healing; mesenchymal stromal cell; angiogenesis; bone marrow cell; luminescence;
D O I：
10.1117/1.3042266
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We investigate the relationship between the fate and healing effect of transplanted mesenchymal stromal cells (MSCs) in a rat diabetic skin wound model. Rats are treated with streptozotocin to induce diabetic conditions. A full-thickness skin defect is surgically made on the head of diabetic rats, and covered with an artificial dermis impregnated with either bone marrow cells (BMCs) or bone-marrow-derived MSCs from firefly luciferase (luc) transgenic (Tg) rats. Wound healing is evaluated using planimetry and immunohistochemistry, and the fate of transplanted MSCs is determined using in-vivo luminescent imaging. The diabetic wound treated with MSCs-impregnated artificial dermis is significantly smaller than that treated with artificial dermis alone at 1 week postoperation. Photons of luc+ MSCs are detected at the transplanted site during healing (3 weeks), whereas those of luc+ MSCs are depleted only after 1 week postimplantation. Immunohistochemistry at the healing site treated with MSCs demonstrates that CD31+ vessels increase with expression of vascular endothelial growth factor, suggesting that MSCs accelerate angiogenesis. These findings suggest that transplanted MSCs could be retained at wound sites during the healing process in a diabetic rat model, and subsequently promote wound healing through angiogenesis. (c) 2008 Society of Photo-Optical Instrumentation Engineers. [DOI: 10.1117/1.3042266]
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页数：10
相关论文
共 42 条
[1]   Nucleofection is an efficient nonviral transfection technique for human bone marrow-derived mesenchymal stem cells [J].
Aluigi, Michela ;
Fogli, Miriam ;
Curti, Antonio ;
Isidori, Alessandro ;
Gruppioni, Elisa ;
Chiodoni, Claudia ;
Colombo, Mario P. ;
Versura, Piera ;
D'Errico-Grigioni, Antonia ;
Ferri, Elisa ;
Baccarani, Michele ;
Lemoli, Roberto M. .
STEM CELLS, 2006, 24 (02) :454-461
[2]   Isolation of putative progenitor endothelial cells for angiogenesis [J].
Asahara, T ;
Murohara, T ;
Sullivan, A ;
Silver, M ;
vanderZee, R ;
Li, T ;
Witzenbichler, B ;
Schatteman, G ;
Isner, JM .
SCIENCE, 1997, 275 (5302) :964-967
[3]   New technologies for burn wound closure and healing - Review of the literature [J].
Atiyeh, BS ;
Hayek, SN ;
Gunn, SW .
BURNS, 2005, 31 (08) :944-956
[4]   Participation of bone marrow derived cells in cutaneous wound healing [J].
Badiavas, EV ;
Abedi, M ;
Butmarc, J ;
Falanga, V ;
Quesenberry, P .
JOURNAL OF CELLULAR PHYSIOLOGY, 2003, 196 (02) :245-250
[5]   Treatment of chronic wounds with bone marrow-derived cells [J].
Badiavas, EV ;
Falanga, V .
ARCHIVES OF DERMATOLOGY, 2003, 139 (04) :510-516
[6]   Treatment of diabetic wounds with fetal murine mesenchymal stromal cells enhances wound closure [J].
Badillo, Andrea T. ;
Redden, Robert A. ;
Zhang, Liping ;
Doolin, Edward J. ;
Liechty, Kenneth W. .
CELL AND TISSUE RESEARCH, 2007, 329 (02) :301-311
[7]   The global burden of diabetic foot disease [J].
Boulton, AJM ;
Vileikyte, L ;
Ragnarson-Tennvall, G ;
Apelqvist, J .
LANCET, 2005, 366 (9498) :1719-1724
[8]   From marrow to brain: Expression of neuronal phenotypes in adult mice [J].
Brazelton, TR ;
Rossi, FMV ;
Keshet, GI ;
Blau, HM .
SCIENCE, 2000, 290 (5497) :1775-1779
[9]   Wound healing and its impairment in the diabetic foot [J].
Falanga, V .
LANCET, 2005, 366 (9498) :1736-1743
[10]  
FRIEDENSTEIN AJ, 1974, EXP HEMATOL, V2, P83
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