Influence of DNA methylation on positioning and DNA flexibility of nucleosomes with pericentric satellite DNA

被引：20

作者：

Osakabe, Akihisa ^{[1
]}

Adachi, Fumiya ^{[1
]}

Arimura, Yasuhiro ^{[1
]}

Maehara, Kazumitsu ^{[2
]}

Ohkawa, Yasuyuki ^{[2
]}

Kurumizaka, Hitoshi ^{[1
]}

机构：

[1] Waseda Univ, Struct Biol Lab, Grad Sch Adv Sci & Engn, Shinjuku Ku, Tokyo 1628480, Japan

[2] Kyushu Univ, Fac Med, Dept Adv Med Initiat, Fukuoka 8128582, Japan

来源：

OPEN BIOLOGY | 2015年 / 5卷 / 10期

关键词：

chromatin; nucleosome; pericentric heterochromatin; DNA methylation; CRYSTAL-STRUCTURE; CPG-ISLANDS; BINDING PROTEINS; CORE PARTICLE; CANCER; HYPOMETHYLATION; EXPRESSION; COMPACTION; STABILITY; CHROMATIN;

D O I：

10.1098/rsob.150128

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

DNA methylation occurs on CpG sites and is important to form pericentric heterochromatin domains. The satellite 2 sequence, containing seven CpG sites, is located in the pericentric region of human chromosome 1 and is highly methylated in normal cells. In contrast, the satellite 2 region is reportedly hypomethylated in cancer cells, suggesting that the methylation status may affect the chromatin structure around the pericentric regions in tumours. In this study, we mapped the nucleosome positioning on the satellite 2 sequence in vitro and found that DNA methylation modestly affects the distribution of the nucleosome positioning. The micrococcal nuclease assay revealed that the DNA end flexibility of the nucleosomes changes, depending on the DNA methylation status. However, the structures and thermal stabilities of the nucleosomes are unaffected by DNA methylation. These findings provide new information to understand how DNA methylation functions in regulating pericentric heterochromatin formation and maintenance in normal and malignant cells.

引用

页数：9

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