Structure elucidation and biosynthesis gene cluster organization of the O-antigen of Escherichia coli O170

被引：5
|
作者：
Shashkov, Alexander S. [1 ]
Wang, Tianwei [2 ]
Perepelov, Andrei V. [1 ]
Weintraub, Andrej [3 ]
Lu, Bin [2 ]
Widmalm, Goran [4 ]
Knirel, Yuriy A. [1 ]
机构：
[1] Russian Acad Sci, ND Zelinsky Inst Organ Chem, Moscow 119991, Russia
[2] Nankai Univ, TEDA, TEDA Sch Biol Sci & Biotechnol, Tianjin 300457, Peoples R China
[3] Karolinska Inst, Karolinska Univ Hosp, Div Clin Microbiol, Dept Lab Med, S-14186 Stockholm, Sweden
[4] Stockholm Univ, Arrhenius Lab, Dept Organ Chem, S-10691 Stockholm, Sweden
来源：
CARBOHYDRATE RESEARCH | 2015年 / 417卷
基金：
中国国家自然科学基金; 俄罗斯科学基金会; 瑞典研究理事会; 对外科技合作项目（国际科技项目）;
关键词：
Escherichia coli; Lipopolysaccharide; O-polysaccharide; Bacterial polysaccharide structure; O-antigen gene cluster; POLYSACCHARIDE;
D O I：
10.1016/j.carres.2015.08.013
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Enterotoxigenic Escherichia coli are causative agents of diarrhea in humans as well as animals, and E. coli O170 belongs to this virotype. Upon mild acid degradation of the lipopolysaccharide of E. coli O170, the branched O-polysaccharide chain was partially cleaved at beta-D-glactofuranosidic linkages to give multiple products, including a linear tetrasaccharide and oligomers thereof. Studies of the acid degradation products and O-deacylated lipopolysaccharide by 1D and 2D H-1 and C-13 NMR spectroscopy enabled elucidation of the following O-polysaccharide structure: -> 4)-beta-D-GlcpNAc-(1 -> 4)-beta-D-GlcpA-(1 -> 3)-beta-D-Galf-(1 -> 3)-beta-D-GlcNAc-(1 -> [GRAPHICS] beta-D-Galf Functions of genes in the O-antigen biosynthesis gene cluster were tentatively assigned and found to be in agreement with the O-polysaccharide structure. (C) 2015 Elsevier Ltd. All rights reserved.
引用
收藏
页码：11 / 14
页数：4
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