Electrochemical monitoring of reactive oxygen/nitrogen species and redox balance in living cells

被引：23

作者：

Malferrari, Marco ^{[1
]}

Becconi, Maila ^{[1
]}

Rapino, Stefania ^{[1
]}

机构：

[1] Univ Bologna, Dept Chem Giacomo Ciamician, Via F Selmi 2, I-40126 Bologna, Italy

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2019年 / 411卷 / 19期

关键词：

Electrochemical techniques; Reactive oxygen species; Reactive nitrogen species; Cellular redox balance; Living cells; Ultramicroelectrodes; HYDROGEN-PEROXIDE; REAL-TIME; SINGLE-CELL; OXIDATIVE STRESS; NADPH OXIDASE; OXYGEN; MICROSCOPY; FIBROBLASTS; RELEASE; ROS;

D O I：

10.1007/s00216-019-01734-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Levels of reactive oxygen species (ROS) and reactive nitrogen species (RNS) in cells and cell redox balance are of great interest in live cells as they are correlated to several pathological and physiological conditions of living cells. ROS and RNS detection is limited due to their spatially restricted abundance: they are usually located in sub-cellular areas (e.g., in specific organelles) at low concentration. In this work, we will review and highlight the electrochemical approach to this bio-analytical issue. Combining electrochemical methods and miniaturization strategies, specific, highly sensitive, time, and spatially resolved measurements of cellular oxidative stress and redox balance analysis are possible.

引用

页码：4365 / 4374

页数：10

共 66 条

[61] Deconvoluting Topography and Spatial Physiological Activity of Live Macrophage Cells by Scanning Electrochemical Microscopy in Constant-Distance Mode [J].