High pressure modulation of Escherichia coli DNA gyrase activity

被引：7

作者：

Chilukuri, LN

Fortes, PAG

Bartlett, DH

机构：

[1] UNIV CALIF SAN DIEGO, SCRIPPS INST OCEANOG, CTR MARINE BIOTECHNOL & BIOMED, LA JOLLA, CA 92093 USA

[2] UNIV CALIF SAN DIEGO, DEPT BIOL, LA JOLLA, CA 92093 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1997年 / 239卷 / 02期

关键词：

D O I：

10.1006/bbrc.1997.7503

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Elevated pressures greater than 551 bar were found to inhibit the DNA supercoiling activity of Escherichia coli DNA gyrase. A large fraction af the inhibitory effect could be reproduced by preincubation of the enzyme at high pressure prior to enzymatic assay at 1 bar. It is proposed that elevated pressure influences gyrase structure, most likely by promoting the dissociation of its subunits, however, it is also possible that effects on enzyme activity exist. (C) 1997 Academic Press.

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页码：552 / 556

页数：5

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