THE DELETION OF Ffh IN Escherichia coli INDUCES ADVERSE PHYSIOLOGICAL AND MORPHOLOGICAL CHANGES

被引：0
|
作者：
Dong, H. J. [1 ]
Shen, X. L. [2 ]
Jiang, J. Y. [1 ]
Li, Y. Q. [2 ]
机构：
[1] Lunan Pharmaceut Co, Inst Drug Res, Linyi 276005, Peoples R China
[2] Zhejiang Univ, Coll Life Sci, Hangzhou 310058, Zhejiang, Peoples R China
来源：
BIOLOGICHESKIE MEMBRANY | 2009年 / 26卷 / 03期
基金：
芬兰科学院; 中国国家自然科学基金;
关键词：
Ffh; Escherichia coli; membrane proteins; SIGNAL-RECOGNITION PARTICLE; PROTEIN EXPORT; 4.5S RNA; GENE; RIBONUCLEOPROTEIN; TRANSLOCATION; RECEPTOR; MUTANT;
D O I：
暂无
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
The Escherichia coli Ffh protein is homologous to the SRP54 subunit of the eukaryotic signal recognition particle (SRP) that is involved in targeting and translocation of membrane proteins. The functions of Ffth in E. coli were investigated using the mutant with the Ffh deficiency. The mutant showed lower growth rate at 30 degrees C and rapidly lost viability at the non-permissive temperature of 42 degrees C. In addition, the amount of the total membrane proteins decreased sharply in the mutant. The mutant cells cultured at either 30 or 42 degrees C appeared to have an elongated shape as compared to the wild type cells. Transmission electron microscopy revealed that the membrane layer of the mutant cells was thinner than that of the wild type cells.
引用
收藏
页码：201 / 205
页数：5
相关论文
共 50 条
  • [31] Nitrogen Starvation Induces Persister Cell Formation in Escherichia coli
    Brown, Daniel R.
    JOURNAL OF BACTERIOLOGY, 2019, 201 (03)
  • [32] Hemolysin from Escherichia coli induces oxidative stress in blood
    Luis Baronetti, Jose
    Angel Villegas, Natalia
    Aiassa, Virginia
    Gabriela Paraje, Maria
    Albesa, Ines
    TOXICON, 2013, 70 : 15 - 20
  • [33] Supercritical CO2 Induces Marked Changes in Membrane Phospholipids Composition in Escherichia coli K12
    Tamburini, Sabrina
    Anesi, Andrea
    Ferrentino, Giovanna
    Spilimbergo, Sara
    Guella, Graziano
    Jousson, Olivier
    JOURNAL OF MEMBRANE BIOLOGY, 2014, 247 (06) : 469 - 477
  • [34] Extracellular Recombinant Protein Production From an Escherichia coli lpp Deletion Mutant
    Shin, Hyun-Dong
    Chen, Rachel R.
    BIOTECHNOLOGY AND BIOENGINEERING, 2008, 101 (06) : 1288 - 1296
  • [35] Stationary phase deletions in Escherichia coli I -: Evidence for a new deletion pathway
    Balbinder, E
    MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS, 2001, 479 (1-2) : 19 - 36
  • [36] Growth inhibition by ppc deletion is rescued by isocitrate dehydrogenase mutations in Escherichia coli
    Toya, Yoshihiro
    Imada, Tatsumi
    Ishibashi, Mai
    Kawamoto, Yuichi
    Isshiki, Kinuka
    Shibai, Atsushi
    Furusawa, Chikara
    Shimizu, Hiroshi
    FEMS MICROBIOLOGY LETTERS, 2025, 372
  • [37] Detecting Photoactivatable Cre-mediated Gene Deletion Efficiency in Escherichia coli
    Koganezawa, Yuta
    Wakamoto, Yuichi
    Sato, Moritoshi
    Umetani, Miki
    BIO-PROTOCOL, 2023, 13 (11):
  • [38] Escherichia coli: Physiological Clues Which Turn On the Synthesis of Antimicrobial Molecules
    Paquette, Sarah-Jo
    Reuter, Tim
    VETERINARY SCIENCES, 2020, 7 (04) : 1 - 14
  • [39] Inflammatory and pathological changes in Escherichia coli infected mice
    Long, Nana
    Deng, Jingzhu
    Qiu, Min
    Zhang, Yanjiao
    Wang, Yuzhen
    Guo, Wei
    Dai, Min
    Lin, Lin
    HELIYON, 2022, 8 (12)
  • [40] Morphological and Transcriptional Responses to CRISPRi Knockdown of Essential Genes in Escherichia coli
    Silvis, Melanie R.
    Rajendram, Manohary
    Shi, Handuo
    Osadnik, Hendrik
    Gray, Andrew N.
    Cesar, Spencer
    Peters, Jason M.
    Hearne, Cameron C.
    Kumar, Parth
    Todor, Horia
    Huang, Kerwyn Casey
    Gross, Carol A.
    MBIO, 2021, 12 (05):
12345