THE DELETION OF Ffh IN Escherichia coli INDUCES ADVERSE PHYSIOLOGICAL AND MORPHOLOGICAL CHANGES

被引：0
|
作者：
Dong, H. J. [1 ]
Shen, X. L. [2 ]
Jiang, J. Y. [1 ]
Li, Y. Q. [2 ]
机构：
[1] Lunan Pharmaceut Co, Inst Drug Res, Linyi 276005, Peoples R China
[2] Zhejiang Univ, Coll Life Sci, Hangzhou 310058, Zhejiang, Peoples R China
来源：
BIOLOGICHESKIE MEMBRANY | 2009年 / 26卷 / 03期
基金：
芬兰科学院; 中国国家自然科学基金;
关键词：
Ffh; Escherichia coli; membrane proteins; SIGNAL-RECOGNITION PARTICLE; PROTEIN EXPORT; 4.5S RNA; GENE; RIBONUCLEOPROTEIN; TRANSLOCATION; RECEPTOR; MUTANT;
D O I：
暂无
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
The Escherichia coli Ffh protein is homologous to the SRP54 subunit of the eukaryotic signal recognition particle (SRP) that is involved in targeting and translocation of membrane proteins. The functions of Ffth in E. coli were investigated using the mutant with the Ffh deficiency. The mutant showed lower growth rate at 30 degrees C and rapidly lost viability at the non-permissive temperature of 42 degrees C. In addition, the amount of the total membrane proteins decreased sharply in the mutant. The mutant cells cultured at either 30 or 42 degrees C appeared to have an elongated shape as compared to the wild type cells. Transmission electron microscopy revealed that the membrane layer of the mutant cells was thinner than that of the wild type cells.
引用
收藏
页码：201 / 205
页数：5
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