Isolation of viable sperm cells from tobacco (Nicotiana tabacum)

被引：8

作者：

Cao, YJ ^{[1
]}

Reece, A ^{[1
]}

Russell, SD ^{[1
]}

机构：

[1] UNIV OKLAHOMA,DEPT BOT & MICROBIOL,NORMAN,OK 73019

来源：

ZYGOTE | 1996年 / 4卷 / 02期

关键词：

semi-vivo; sperm isolation; Nicotiana tabacum; tobacco;

D O I：

10.1017/S096719940000294X

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Viable sperm cells of Nicotiana tabacum were isolated by the semi-vivo technique. After pollination, excised styles were floated, cut end immersed, in a solution of 15% sucrose with 0.01% boric acid and 0.03% Ca(NO3)2 at 27 degrees C in a growth chamber until pollen tubes emerged. After sperm cells were formed (at least 8 h after pollination) tubes were immersed in a 9% mannitol solution. In this solution, sperm cells are nearly ellipsoidal and retain viability for over 6 h.

引用

页码：81 / 84

页数：4

共 11 条

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